Difference between revisions of "Part:BBa K3889020"
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P22 c2 repressor is from Salmonella P22 phage, which binds to its binding site (<partinfo>BBa_K3889080</partinfo> to <partinfo>BBa_K3889091</partinfo>) as a dimer leading to inhibition of the gene on which its binding site is present [1]. | P22 c2 repressor is from Salmonella P22 phage, which binds to its binding site (<partinfo>BBa_K3889080</partinfo> to <partinfo>BBa_K3889091</partinfo>) as a dimer leading to inhibition of the gene on which its binding site is present [1]. | ||
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This is a modified version of <partinfo>BBa_C0053</partinfo> specific to our project's assembly. | This is a modified version of <partinfo>BBa_C0053</partinfo> specific to our project's assembly. | ||
The following modifications were made: | The following modifications were made: | ||
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*LVA tag Removed | *LVA tag Removed | ||
*barcodes Removed | *barcodes Removed | ||
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| + | [[File:T--IISER-Tirupati India--P22 repression.png|centre]] | ||
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Latest revision as of 00:17, 20 October 2021
P22 c2 repressor
P22 c2 repressor is from Salmonella P22 phage, which binds to its binding site (BBa_K3889080 to BBa_K3889091) as a dimer leading to inhibition of the gene on which its binding site is present [1].
This is a modified version of BBa_C0053 specific to our project's assembly. The following modifications were made:
- Spa1 site Removed
- LVA tag Removed
- barcodes Removed
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1. Watkins, D., Hsiao, C., Woods, K. K., Koudelka, G. B., & Williams, L. D. (2008). P22 c2 Repressor−Operator Complex: Mechanisms of Direct and Indirect Readout. Biochemistry, 47(8), 2325–2338. https://doi.org/10.1021/bi701826f