Difference between revisions of "Part:BBa K3714001"
 
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<partinfo>BBa_K3714001 short</partinfo>
 
<partinfo>BBa_K3714001 short</partinfo>
  
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A gardiquimod biosensor with a loss-of-function mutation G12A eliminating its self-cleavage activity.  
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The template of a theophylline biosensor. Cleave itself without theophylline. Two A-rich sequences were attached at both terminal to minimal the unwanted interaction.  
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3714001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3714001 SequenceAndFeatures</partinfo>
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<center>[[File:K3714001.png]]</center>
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<center><b>Figure.1 | Verification of biosensor theo-AAG/AAAAA. </b> An in vitro transcription system was employed to test if this biosensor can response theophylline signal. The product was run on a denatured PAGE. </center>
  
 
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Latest revision as of 23:07, 20 October 2021


Theophylline biosensor AAG-AAAAA

The template of a theophylline biosensor. Cleave itself without theophylline. Two A-rich sequences were attached at both terminal to minimal the unwanted interaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
K3714001.png
Figure.1 | Verification of biosensor theo-AAG/AAAAA. An in vitro transcription system was employed to test if this biosensor can response theophylline signal. The product was run on a denatured PAGE.