Difference between revisions of "Part:BBa K3861024"

 
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<partinfo>BBa_K3861024 short</partinfo>
 
<partinfo>BBa_K3861024 short</partinfo>
  
Plldr-sicP-spt-Pep8
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Composite part consisting of <a href="https://parts.igem.org/Part:BBa_K3861024?action=edit">BBa_K3861024</a> and <a href="https://parts.igem.org/Part:BBa_K3861010?action=edit">BBa_K3861010</a>. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Further, due to the SPI T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI T3SS.   
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Composite part consisting of <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861000">BBa_K3861000</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861010">BBa_K3861010</a>. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Further, due to the SPI-1 T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI-1 T3SS.   
 
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:33, 21 October 2021


PlldR-sicP-sptP-Pep8


Composite part consisting of BBa_K3861000 and BBa_K3861010. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Further, due to the SPI-1 T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI-1 T3SS.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 519
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
    Illegal PstI site found at 519
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 519
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]