Difference between revisions of "Part:BBa K3726018"

 
 
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__NOTOC__
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<partinfo>BBa_K3726018 short</partinfo>
  
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This composite part corresponds to a phytobrick compatible MoClo Lv.0 CDS element.  The protein encoded by this part corresponds with a chimeric protein, created by the fusion of two different enzymes using a linker . Within this part, the enzymes CDS_PduP (BBa_K3726004), CDS_Slr1192  (BBa_K3726005).
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The linker used between both parts is FRRRF  (Flexible-Rigid (x3) - Flexible tándem). Which correspond with the “GGGGSEAAAKEAAAKEAAAKGGGGS” aa residues which have demonstrated successful results for enzyme scaffolding, allowing to increase the specific activity of both enzymes within a production pathway, aided by their spatial proximity.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3726018 SequenceAndFeatures</partinfo>

Latest revision as of 21:44, 19 October 2021

CDS_Lv0_BOH1_C_FRRRF

This composite part corresponds to a phytobrick compatible MoClo Lv.0 CDS element. The protein encoded by this part corresponds with a chimeric protein, created by the fusion of two different enzymes using a linker . Within this part, the enzymes CDS_PduP (BBa_K3726004), CDS_Slr1192 (BBa_K3726005).

The linker used between both parts is FRRRF (Flexible-Rigid (x3) - Flexible tándem). Which correspond with the “GGGGSEAAAKEAAAKEAAAKGGGGS” aa residues which have demonstrated successful results for enzyme scaffolding, allowing to increase the specific activity of both enzymes within a production pathway, aided by their spatial proximity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1246
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 252
    Illegal NheI site found at 480
    Illegal PstI site found at 1246
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1641
    Illegal BglII site found at 1758
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1246
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1246
  • 1000
    COMPATIBLE WITH RFC[1000]