Difference between revisions of "Part:BBa K3771020"
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<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:60%;"> | <img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:60%;"> | ||
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− | <p align="center">Fig.1 Taurine pathways in <i>E. coli</i></p> | + | <p align="center">Fig. 1. Taurine pathways in <i>E. coli</i></p> |
<br><b style="font-size:1.3rem">Usage</b> | <br><b style="font-size:1.3rem">Usage</b> | ||
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<img src="https://2021.igem.org/wiki/images/f/fa/T--NCKU_Tainan--colony_jju.jpg | <img src="https://2021.igem.org/wiki/images/f/fa/T--NCKU_Tainan--colony_jju.jpg | ||
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<p align="center">Fig. 2. Colony PCR confirmation of the construction</p> | <p align="center">Fig. 2. Colony PCR confirmation of the construction</p> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 03:21, 22 October 2021
PpspA-JJU
Description
This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, JJU.
Biology
Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria.[1]Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of JJU. JJU converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway.
Fig. 1. Taurine pathways in E. coli
Usage
We ligased the jju fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.
Characterization
Fig. 2. Colony PCR confirmation of the construction
Reference
1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x https://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 12
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]