Difference between revisions of "Part:BBa K3794004"

Latest revision as of 18:21, 21 October 2021

ChABC CDS

Chondroitinase ABC (ChABC) is a bacterial enzyme secreted by Proteus vulgaris , it degrades the glycosaminoglycan (GAG) chains of chondroitin sulfate proteo-glycans (CSPGs) to allow for axonal regeneration following spinal cord injuries.

This ChABC part is derived from PDB ID: 1HN0, and also encodes a 6xHis tag and a TEV cleavage site for protein purification via a Ni-NTA column. Upstream of the 6xHis tag, there is a start codon to allow for transcriptional initiation.

ChABC can be administered in the form of microinjections to the glial scar that is formed during a spinal cord injury, to degrade GAG chains from CSPGs - which are inhibitory molecules to axonal regeneration.

Due to the thermal instability of native ChABC at physiological temperatures, 8 point mutations to confer thermostability at 37C were introduced before synthesis of part in its composite form (BBa_K3794005) by IDT.

These mutations are as follows:

K194E
A228K
S274P
N288D
S343N
K654D
R670T
Q781E

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2754
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Chondroitinase ABC (ChABC) is a bacterial enzyme secreted by <i> Proteus vulgaris </i>, it degrades the glycosaminoglycan (GAG) chains of chondroitin sulfate proteo-glycans (CSPGs) to allow for axonal regeneration following spinal cord injuries.
-This ChABC part is derived from PDB ID: 1HN0, and also encodes a 6xHis tag and a TEV cleavage site for protein purification via a Ni-NTA column.
+This ChABC part is derived from PDB ID: 1HN0, and also encodes a 6xHis tag and a TEV cleavage site for protein purification via a Ni-NTA column. Upstream of the 6xHis tag, there is a start codon to allow for transcriptional initiation.
 ChABC can be administered in the form of microinjections to the glial scar that is formed during a spinal cord injury, to degrade GAG chains from CSPGs - which are inhibitory molecules to axonal regeneration.
-Due to the thermal instability of native ChABC at physiological temperatures, 8 point mutations to confer thermostability at 37C were introduced before synthesis of part in its composite form by IDT.
+Due to the thermal instability of native ChABC at physiological temperatures, 8 point mutations to confer thermostability at 37C were introduced before synthesis of part in its composite form (BBa_K3794005) by IDT.
 These mutations are as follows: