Difference between revisions of "Part:BBa K3732003"
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==Contribution from iGEM21-FAFU-China==
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==Contribution From FAFU-China 2021==
  
 
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Latest revision as of 19:04, 19 October 2021


Test element for optical control element

The COMBINATION of RFP and light control element BBa_K3732000 is used to quickly detect the expression of target gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The PCR results showed that we successfully assembled BBa_K3732002, BBa_K3732000 and BBa_K3732001 together. Since the vector used also contains BBa_K3732001, the yeast grows well on the corresponding resistant plates.


Contribution From FAFU-China 2021

This is a composite component composed of BBa_K3732002, BBa_K3732000 and BBa_K3732001.

We fused the DNA sequence of BBa_K3732000 with the 3 'end of the DNA sequence of the red fluorescent protein in the plasmid to study the biological function of BBa_K3732000 in the early stage. Meanwhile, BBa_K3732000 and kanMX were connected in series in the plasmid. After the element was tested, primers with upstream and downstream homologous arms of the termination codon of the target gene were designed to clone 5 'mRFP1 to kanMX 3' for homologous recombination. As a result, BBa_K3732000 and KanMX were added to the loci of the target gene.