Difference between revisions of "Part:BBa K3771011"
 
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<br><br><b style="font-size:1.3rem">Characterization  
 
<br><br><b style="font-size:1.3rem">Characterization  
 
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<br><br>The <i>ompA</i> promoter sequence was amplified from <i>E. coli</i> MG1655 and cloned into our plasmid. Agarose gel electrophoresis results confirming the construction with the <i>ompA</i> promoter are shown in Figure 1.
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<br><br>The <i>ompA</i> promoter sequence was amplified from <i>E. coli</i> MG1655 and cloned into our plasmid. Agarose gel electrophoresis results confirming the construction with the <i>ompA</i> promoter are shown in Fig. 1.
  
  
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<img src="https://2021.igem.org/wiki/images/b/b0/T--NCKU_Tainan--pcr_ompA_promoter.jpg" style="width:40%;">
 
<img src="https://2021.igem.org/wiki/images/b/b0/T--NCKU_Tainan--pcr_ompA_promoter.jpg" style="width:40%;">
 
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   <p align="center">Fig. 1. Confirmation of <i>P<sub>ompA</sub></i> fragment by PCR. M: Marker; Lane 1: <i>P<sub>ompA</sub></i> (309 bp)</p>
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   <p align="center">Fig. 1. Confirmation of <i>P<sub>ompA</sub></i> fragment by PCR. M: Marker; Lane 1: <i>P<sub>ompA</sub></i> (303 bp)</p>
 
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<br><br><b style="font-size:1.3rem">References  
 
<br><br><b style="font-size:1.3rem">References  
 
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<br>[1]Cole ST, Bremer E, Hindennach I, Henning U. Characterisation of the promoters for the ompA gene which encodes a major outer membrane protein of Escherichia coli. Molecular and General Genetics MGG. 1982;188(3):472-479. doi:10.1007/bf00330051
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<br>[2]Escherichia coli K-12 substr. MG1655 ompA. Biocyc.org. Published 2021. Accessed September 8, 2021.  
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<br>1. Cole ST, Bremer E, Hindennach I, Henning U. Characterisation of the promoters for the ompA gene which encodes a major outer membrane protein of Escherichia coli. Molecular and General Genetics MGG. 1982;188(3):472-479. doi:10.1007/bf00330051
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<br>[3]Udekwu KI. Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Genes & Development. 2005;19(19):2355-2366. doi:10.1101/gad.354405
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<br>2. Escherichia coli K-12 substr. MG1655 ompA. Biocyc.org. Published 2021. Accessed September 8, 2021.  
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<br>3. Udekwu KI. Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Genes & Development. 2005;19(19):2355-2366. doi:10.1101/gad.354405
  
  

Latest revision as of 02:04, 22 October 2021


ompA promoter



Description

ompA promoter is a constitutive promoter that drives the expression of OmpA transmembrane protein in E. coli and other enterobacteria [1].

Biology

The ompA promoter is -35/-10 region upstream of the ompA gene in E. coli MG1655. OmpA protein is regulated by two overlapping promoters ompAp1 and ompAp2 [1,2].

Usage

ompA promoter was used to regulate the expression of OmpA/OprF chimeric protein in the IFN-γ sensing system.

Characterization

The ompA promoter sequence was amplified from E. coli MG1655 and cloned into our plasmid. Agarose gel electrophoresis results confirming the construction with the ompA promoter are shown in Fig. 1.



Fig. 1. Confirmation of PompA fragment by PCR. M: Marker; Lane 1: PompA (303 bp)



References

1. Cole ST, Bremer E, Hindennach I, Henning U. Characterisation of the promoters for the ompA gene which encodes a major outer membrane protein of Escherichia coli. Molecular and General Genetics MGG. 1982;188(3):472-479. doi:10.1007/bf00330051
2. Escherichia coli K-12 substr. MG1655 ompA. Biocyc.org. Published 2021. Accessed September 8, 2021.
3. Udekwu KI. Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Genes & Development. 2005;19(19):2355-2366. doi:10.1101/gad.354405




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]