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| | + | __NOTOC__ |
| | + | <partinfo>BBa_K3734002 short</partinfo> |
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| | + | GIP is a promoter that can be induced by glucose. We first use it in 293T cell. |
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| | <html> | | <html> |
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| − | <meta charset="utf-8" /> | + | |
| − | <meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no" />
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| − | <meta name="csu_china" content="csu_china" />
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| − | <title>Team:csu_china</title>
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| − | <!-- Favicon-->
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| − | <link rel="icon" type="image/x-icon" href="assets/favicon.ico" />
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| − | <!-- Font Awesome icons (free version)-->
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| − | <script src="https://use.fontawesome.com/releases/v5.15.3/js/all.js" crossorigin="anonymous"></script>
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| − | <!-- Google fonts-->
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| − | <link href="https://fonts.googleapis.com/css?family=Montserrat:400,700" rel="stylesheet" type="text/css" />
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| − | <link href="https://fonts.googleapis.com/css?family=Roboto+Slab:400,100,300,700" rel="stylesheet" type="text/css" />
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| − | <!-- Core theme CSS (includes Bootstrap)-->
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| − | <script src="http://code.jquery.com/jquery-latest.js"></script>
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| − | <script src="https://2021.igem.org/wiki/index.php?title=Template:CSU_CHINA/JS/script&action=raw&ctype=text/javascript"></script>
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| − | <script src="https://2021.igem.org/wiki/index.php?title=Template:CSU_CHINA/JS/scripts&action=raw&ctype=text/javascript"></script>
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| | </head> | | </head> |
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| | <body> | | <body> |
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| − | font-family: 'Source Sans Pro', -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, 'Helvetica Neue', Arial, sans-serif;
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| − | font-size: 16px;
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| − | word-spacing: 1px;
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| − | -ms-text-size-adjust: 100%;
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| − | -webkit-text-size-adjust: 100%;
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| − | -moz-osx-font-smoothing: grayscale;
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| − | :root {
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| − | --primary: #074E9F;
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| − | --primary-transparent: rgba(7, 78, 159, 0.5);
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| − | --blue: #6DBFFF;
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| − | --yellow: #FFC93C;
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| − | --dark: #0d2b4d;
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| − | --title-color: #004290;
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| − | --text-color: #00244E;
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| − | #navbar {
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| − | position: absolute;
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| − | left: 0;
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| − | width: 100%;
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| − | /* background-color: */
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| − | background: var(--primary-transparent);
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| − | #navbar>.logo {
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| − | width: 100%;
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| − | padding: .5rem 0 0 0;
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| − | text-align: center;
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| − | #collapse {
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| − | #content {
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| − | width: 100%;
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| − | #top_title {
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| − | display: none;
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| − | #collapse>.navbar-list {
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| − | flex-shrink: 0;
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| − | padding: .5rem;
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| − | display: flex;
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| − | flex-shrink: 0;
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| − | text-align: center;
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| − | flex-direction: row;
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| − | vertical-align: center;
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| − | align-items: center;
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| − | text-align: center;
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| − | #collapse>.mainlist {
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| − | flex: 1;
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| − | /* overflow-y: hidden;
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| − | /* overflow: auto; */
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| − | #collapse>.navbar-list>.navbar-list__item {
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| − | font-size: .9rem;
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| − | line-height: 1.5rem;
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| − | color: #ffffff;
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| − | font-weight: bold;
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| − | padding: .3rem 1rem;
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| − | /* flex: 1; */
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| − | text-decoration: none;
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| − | margin: 0 .3rem;
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| − | font-family: San Francisco Display;
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| − | transition: all .3s;
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| − | border-radius: 2rem;
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| − | position: relative;
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| − | cursor: pointer;
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| − | #collapse>.navbar-list>.navbar-list__item:hover {
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| − | #collapse>.navbar-list>.navbar-list__item .navbar-list__menu {
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| − | position: absolute;
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| − | top: 2.2rem;
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| − | background-color: #ffffff;
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| − | border-radius: 5px;
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| − | box-shadow: 0 3px 10px -2px rgba(0, 0, 0, 0.1);
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| − | width: 10rem;
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| − | left: calc(50% - 5rem);
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| − | }
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| − | #collapse>.navbar-list>.navbar-list__item .navbar-list__menuItem {
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| − | padding: .5rem 1rem;
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| − | color: #333;
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| − | display: block;
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| − | text-decoration: none;
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| − | cursor: pointer;
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| − | }
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| − | #collapse>.navbar-list>.active,
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| − | #collapse>.navbar-list>.navbar-list__item:hover {
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| − | background-color: var(--yellow);
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| − | color: var(--primary);
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| − | #collapse>.navbar-list>.teamname {
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| − | font-size: 1.5rem;
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| − | color: var(--yellow);
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| − | #logo {
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| − | width: 2.5rem;
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| − | #home {
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| − | background-color: #ffffff;
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| − | #home>.pageHead {
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| − | height: 80vh;
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| − | width: 100%;
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| − | background-position-y: top;
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| − | background-position-x: center;
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| − | background-size: cover;
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| − | background-image: url('/static/images/lab.png');
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| − | display: flex;
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| − | flex-direction: column;
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| − | justify-content: flex-end;
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| − | z-index: 0;
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| − | }
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| − | #home>.pageHead::before {
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| − | content: "";
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| − | position: absolute;
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| − | top: 0;
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| − | left: 0;
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| − | width: 100%;
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| − | height: 80vh;
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| − | background-color: rgba(0, 36, 78, .5);
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| − | z-index: -1;
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| − | }
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| − | #home>.pageHead>.pageHead-content {
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| − | text-align: center;
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| − | display: flex;
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| − | flex-direction: column;
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| − | }
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| − | #home>.pageHead>.pageHead-content>.pageHead-content__title {
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| − | font-size: 5rem;
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| − | color: #ffffff;
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| − | }
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| − | #home>.pageHead>.pageHead-content>.pageHead-content__description {
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| − | margin: 6rem 10% 4rem 10%;
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| − | font-size: 1rem;
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| − | color: #ffffff;
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| − | }
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| − | #home>.pageContent {
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| − | display: flex;
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| − | min-height: 60vh;
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| − | #home>.pageContent>.pageContent-side {
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| − | width: 20%;
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| − | max-width: 200px;
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| − | flex-shrink: 0;
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| − | }
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| − | #navlist {
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| − | background-color: var(--dark);
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| − | padding: 2rem .5rem 4rem .5rem;
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| − | min-height: 55vh;
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| − | border-bottom-right-radius: 2rem;
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| − | position: relative;
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| − | text-align: center;
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| − | #navlist>.pageContent-navlist__head {
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| − | padding: 1rem 0 1rem 0;
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| − | #navlist>.pageContent-navlist__head>.logo {
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| − | margin: 1rem auto;
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| − | width: 3.5rem;
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| − | height: 3.5rem;
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| − | border-radius: 50%;
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| − | background-color: #dfdfdf;
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| − | }
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| − | #navlist>.pageContent-navlist__head>.title {
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| − | color: #ffffff;
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| − | font-size: 1.5rem;
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| − | font-weight: bold;
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| − | }
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| − | #navlist>.pageContent-navlist__item {
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| − | color: #ffffff;
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| − | font-size: 1rem;
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| − | padding: .6rem .1rem;
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| − | margin: .1rem 0;
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| − | text-align: center;
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| − | width: 100%;
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| − | transition: all .3s;
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| − | text-decoration: none;
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| − | border-radius: 3px;
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| − | #navlist>.pageContent-navlist__item:hover,
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| − | #navlist>.active {
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| − | background-color: var(--yellow);
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| − | color: var(--dark);
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| − | #pagecontent {
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| − | padding: 2rem 5%;
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| − | position: relative;
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| − | flex-shrink: 0;
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| − | #pagecontent .pageContent-main__title {
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| − | font-size: 2rem;
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| − | #pagecontent .pageContent-main__subtitle {
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| − | font-size: 1.5rem;
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| − | #pagecontent .pageContent-main__title::after {
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| − | width: 100%;
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| − | height: 3px;
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| − | background-color: var(--dark);
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| − | left: 0;
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| − | bottom: -1rem;
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| − | }
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| − | #pagecontent .pageContent-main__textBox {
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| − | width: 90%;
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| − | margin: 1rem 5% 3rem 5%;
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| − | color: var(--text-color);
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| − | font-size: 1rem;
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| − | line-height: 1.5rem;
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| − | #pagecontent .pageContent-main__textBox p {
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| − | margin-bottom: 1.1rem;
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| − | font-size: 1.1rem;
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| − | line-height: 1.8rem;
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| − | #pagecontent .pageContent-main__textBox .name {
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| − | font-weight: bold;
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| − | #pagecontent .pageContent-main__textBox .image {
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| − | width: 80%;
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| − | margin: 2rem 10%;
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| − | .pageContent-side .mytoc {
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| − | position: -webkit-sticky;
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| − | position: sticky;
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| − | top: 12rem;
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| − | background: transparent;
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| − | padding: 1.5rem;
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| − | border: none;
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| − | .pageContent-side .mytoc .toc_list {
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| − | font-size: .8rem;
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| − | color: #888;
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| − | line-height: 1.5rem;
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| − | }
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| − | .pageContent-side .mytoc .toc_list .toclist__item a {
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| − | color: #888;
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| − | text-decoration: none;
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| − | .pageContent-side .mytoc .toc_list .toclist__item a:hover {
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| − | color: var(--primary)
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| − | }
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| − | @media screen and (max-width: 800px) {
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| − | #home>.screen>#group1>.title {
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| − | width: 100%;
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| − | }
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| − | #navbar>.logo {
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| − | padding: 0.5rem 0;
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| − | #collapse {
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| − | height: 0;
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| − | #home>.pageContent>.pageContent-side {
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| − | display: none;
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| − | }
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| − | #home>.screen>#group1 {
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| − | padding-top: 5vh;
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| − | }
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| − | #home>.pageContent>.pageContent-main>.profilelist>.profile {
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| − | width: calc(100% / 3)
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| − | }
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| − | }
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| − | </style>
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| | <h2 class="pageContent-main__title"> | | <h2 class="pageContent-main__title"> |
| | <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title--> | | <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title--> |
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| | </body> | | </body> |
| | </html> | | </html> |
| | + | <!-- --> |
| | + | <span class='h3bb'>Sequence and Features</span> |
| | + | <partinfo>BBa_K3734002 SequenceAndFeatures</partinfo> |
| | + | |
| | + | |
| | + | <!-- Uncomment this to enable Functional Parameter display |
| | + | ===Functional Parameters=== |
| | + | <partinfo>BBa_K3734002 parameters</partinfo> |
| | + | <!-- --> |
GIP is a promoter that can be induced by glucose. We first use it in 293T cell.
GIP promoter is anticipated in current literature that it can only be used in several certain types of cells like intestine cells, but we proved that it can function will in 293T cells which are usually used in labs.
Fig.1 The model diagram of CHREBP-LUC
We use LUC as report gene, test its real-time luminescence value at 560nm wavelength with specific devices and detect the expression level under different glucose concentration.
GIP promoter is induced by glucose and the expression level rises along with the raise of glucose concentration.
Fig.2 Changes of LUC expression over time after blue light irradiation with different glucose concentrations
