Difference between revisions of "Part:BBa K3805283"
 
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This part is designed to guide dCas9 to silence the function of the promoter pBAD and to inhibit the initiation of downstream genes.In our project, we used this part to suppress the activation of the kill system.The suppression caused by dCas9 is reversible and does not permanently alter the genomic DNA.This sequence is designed and evaluated by the website http://crispr.dfci.harvard.edu/SSC/.
 
This part is designed to guide dCas9 to silence the function of the promoter pBAD and to inhibit the initiation of downstream genes.In our project, we used this part to suppress the activation of the kill system.The suppression caused by dCas9 is reversible and does not permanently alter the genomic DNA.This sequence is designed and evaluated by the website http://crispr.dfci.harvard.edu/SSC/.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:39, 19 October 2021


sgRNA bind to Pbad promoter

This part is designed to guide dCas9 to silence the function of the promoter pBAD and to inhibit the initiation of downstream genes.In our project, we used this part to suppress the activation of the kill system.The suppression caused by dCas9 is reversible and does not permanently alter the genomic DNA.This sequence is designed and evaluated by the website http://crispr.dfci.harvard.edu/SSC/.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]