Difference between revisions of "Part:BBa K3734002"
| (9 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3734002 short</partinfo> | <partinfo>BBa_K3734002 short</partinfo> | ||
| − | GIP is a promoter that can be induced by glucose. | + | GIP is a promoter that can be induced by glucose. We first use it in 293T cell. |
| + | |||
| + | <html> | ||
| + | |||
| + | <head> | ||
| + | |||
| + | </head> | ||
| − | < | + | <body> |
| − | + | <h2 class="pageContent-main__title"> | |
| − | <h2 class="pageContent-main__title"> | + | |
<!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title--> | <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title--> | ||
GIP promoter | GIP promoter | ||
| Line 22: | Line 26: | ||
<div class="pageContent-main__textBox"> | <div class="pageContent-main__textBox"> | ||
<!--all the content must included in this div--> | <!--all the content must included in this div--> | ||
| − | <img | + | <p style="text-align: center;"> |
| + | <img width="400px" src="https://2021.igem.org/wiki/images/8/87/T--CSU_CHINA--tupian5.png"></p> | ||
<!--put image's url here--> | <!--put image's url here--> | ||
<p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of CHREBP-LUC</p> | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of CHREBP-LUC</p> | ||
| Line 44: | Line 49: | ||
2.2 Result | 2.2 Result | ||
</h2> | </h2> | ||
| − | < | + | <p class="pageContent-main__textBox"> |
<!--all the content must included in this div--> | <!--all the content must included in this div--> | ||
<p>GIP promoter is induced by glucose and the expression level rises along with the raise of glucose concentration. | <p>GIP promoter is induced by glucose and the expression level rises along with the raise of glucose concentration. | ||
</p> | </p> | ||
| − | <img | + | <p style="text-align: center;"> |
| + | <img width="400px" src="https://2021.igem.org/wiki/images/5/59/T--CSU_CHINA--tupian6.png"></p> | ||
<!--put image's url here--> | <!--put image's url here--> | ||
<p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Changes of LUC expression over time after blue light irradiation with different glucose concentrations</p> | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Changes of LUC expression over time after blue light irradiation with different glucose concentrations</p> | ||
<!--put text here, <p>content</p>--> | <!--put text here, <p>content</p>--> | ||
| − | + | </div> | |
| − | + | <h2 class="pageContent-main__title pageContent-main__subtitle"> | |
| − | + | <!--class="pageContent-main__title" means it is the sub title--> | |
| − | + | 3.Caution | |
| − | + | </h2> | |
| − | + | <div class="pageContent-main__textBox"> | |
| − | + | <!--all the content must included in this div--> | |
| − | + | <p>Use medium without glucose when paving in case of the effect caused by glucose residue | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | <h2 class="pageContent-main__title pageContent-main__subtitle"> | |
| − | + | <!--class="pageContent-main__title" means it is the sub title--> | |
| − | + | Reference： | |
| − | + | </h2> | |
| − | + | <div class="pageContent-main__textBox"> | |
| − | + | <!--all the content must included in this div--> | |
| − | + | <p>[1]Anthony T. Cheung,Bama Dayanandan,Jamie T. LewisGlucose-Dependent Insulin Release from Genetically Engineered K Cells[J].Science.2000,290:1959-1962 | |
| − | + | </p> | |
| − | + | </div> | |
| + | </body> | ||
| + | </html> | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 08:16, 21 October 2021
GIP promoter
GIP is a promoter that can be induced by glucose. We first use it in 293T cell.
GIP promoter
GIP promoter is anticipated in current literature that it can only be used in several certain types of cells like intestine cells, but we proved that it can function will in 293T cells which are usually used in labs.
1.Pattern diagram
Fig.1 The model diagram of CHREBP-LUC
2.Experiments
2.1 Method
We use LUC as report gene, test its real-time luminescence value at 560nm wavelength with specific devices and detect the expression level under different glucose concentration.
2.2 Result
GIP promoter is induced by glucose and the expression level rises along with the raise of glucose concentration.
Fig.2 Changes of LUC expression over time after blue light irradiation with different glucose concentrations
3.Caution
Use medium without glucose when paving in case of the effect caused by glucose residue
Reference：
[1]Anthony T. Cheung,Bama Dayanandan,Jamie T. LewisGlucose-Dependent Insulin Release from Genetically Engineered K Cells[J].Science.2000,290:1959-1962
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 899
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 216