Difference between revisions of "Part:BBa M10019:Design"

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===Source===
 
===Source===
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pBca1256-K112602
 
  
 
Wobble PCR Jw0019F / Jw0020R      (45 bp, EcoRI/BamHI) <br>
 
Wobble PCR Jw0019F / Jw0020R      (45 bp, EcoRI/BamHI) <br>

Latest revision as of 03:48, 10 May 2009

{˂FLAG˃}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Later


Source

Wobble PCR Jw0019F / Jw0020R (45 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+ 910, L)
Product is pBca1256-K112602 {Flag}


Jw0019F Forward construction of Flag
cgataGAATTCatgAGATCTgactacaaggatgacgacg
Jw0020R Reverse construction of Flag
ccagtGGATCCcttgtcgtcgtcatccttgtagtc

References

Terpe K. (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. 60:523-33.