Difference between revisions of "Part:BBa K3771100"

 
 
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<br><b style="font-size:1.3rem">Description</b>
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<br>SoxR is a 17 kDa transcription factor of the MerR family. The normal <i>in vivo</i> level of SoxR protein is maintained at a low level of 50-100 molecules per cell, forming a dimer in solution. It contains a [2Fe–2S] cluster, which is essential for reversible one-electron oxidation and reduction to regulate its activity. Only when the [2Fe–2S] cluster is fully oxidized (Fe<sup>3+</sup>-Fe<sup>3+</sup>), SoxR becomes a powerful transcription activator of <i>soxS</i> promoter (up to 100-fold). Reducing the SoxR [2Fe–2S] cluster by one electron (Fe<sup>2+</sup>-Fe<sup>3+</sup>) can eliminate the ability to stimulate SoxS expression<sup>[1]</sup>. In response to superoxide-generating agents, SoxR is oxidized and activated, therefore increasing the synthesis of SoxS, which then activates its target gene expression<sup>[2]</sup>.
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<img src="https://2021.igem.org/wiki/images/b/b8/T--NCKU_Tainan--soxRS_regulon.png"​ style="width:50%;">
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<p align="center">Fig.1. The <i>soxRS</i> regulon. The <i>soxRS</i> regulon includes <i>soxR</i> and <i>soxS</i> genes. The <i>soxR</i> gene is expressed innately in cells and SoxR is activated in response to superoxide-generating agents and nitric oxide (NO). Oxidized SoxR acts as a transcriptional activator of <i>soxS</i> gene; SoxS, afterward, enhances the expression of antioxidant genes. Abbreviation: G6PD, glucose-6-P-dehydrogenase<sup>[1]</sup>.
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===Usage and Biology===
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<br><b style="font-size:1.3rem">Usage and Biology</b>
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<br>SoxR protein was used to regulate the expression of taurine-producing enzymes. When the [2Fe–2S] cluster is fully oxidized, SoxR acts as a strong transcription activator of <i>soxS</i> promoter<sup>[1]</sup>. The sequence of this basic part is the reverse complement of <i>soxR</i> gene from <i>E. coli</i>.
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<br><b style="font-size:1.3rem">References</b>
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<br>1. Pomposiello PJ, Demple B. Redox-operated genetic switches: the SoxR and OxyR transcription factors. <i>Trends Biotechnol</i>. 2001;19(3):109-114. doi:10.1016/s0167-7799(00)01542-0
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<br>2. Koo MS, Lee JH, Rah SY, et al. A reducing system of the superoxide sensor SoxR in Escherichia coli. EMBO J. 2003;22(11):2614-2622. doi:10.1093/emboj/cdg252
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Latest revision as of 18:32, 19 October 2021


SoxR


Description


SoxR is a 17 kDa transcription factor of the MerR family. The normal in vivo level of SoxR protein is maintained at a low level of 50-100 molecules per cell, forming a dimer in solution. It contains a [2Fe–2S] cluster, which is essential for reversible one-electron oxidation and reduction to regulate its activity. Only when the [2Fe–2S] cluster is fully oxidized (Fe3+-Fe3+), SoxR becomes a powerful transcription activator of soxS promoter (up to 100-fold). Reducing the SoxR [2Fe–2S] cluster by one electron (Fe2+-Fe3+) can eliminate the ability to stimulate SoxS expression[1]. In response to superoxide-generating agents, SoxR is oxidized and activated, therefore increasing the synthesis of SoxS, which then activates its target gene expression[2].


Fig.1. The soxRS regulon. The soxRS regulon includes soxR and soxS genes. The soxR gene is expressed innately in cells and SoxR is activated in response to superoxide-generating agents and nitric oxide (NO). Oxidized SoxR acts as a transcriptional activator of soxS gene; SoxS, afterward, enhances the expression of antioxidant genes. Abbreviation: G6PD, glucose-6-P-dehydrogenase[1].



Usage and Biology


SoxR protein was used to regulate the expression of taurine-producing enzymes. When the [2Fe–2S] cluster is fully oxidized, SoxR acts as a strong transcription activator of soxS promoter[1]. The sequence of this basic part is the reverse complement of soxR gene from E. coli.



References


1. Pomposiello PJ, Demple B. Redox-operated genetic switches: the SoxR and OxyR transcription factors. Trends Biotechnol. 2001;19(3):109-114. doi:10.1016/s0167-7799(00)01542-0

2. Koo MS, Lee JH, Rah SY, et al. A reducing system of the superoxide sensor SoxR in Escherichia coli. EMBO J. 2003;22(11):2614-2622. doi:10.1093/emboj/cdg252


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]