Difference between revisions of "Part:BBa M10019:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_M10019 short</partinfo> | <partinfo>BBa_M10019 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | Wobble PCR Jw0019F / Jw0020R (45 bp, EcoRI/BamHI) | + | Wobble PCR Jw0019F / Jw0020R (45 bp, EcoRI/BamHI) <br> |
| − | + | Sub into pBca1256 (EcoRI/BamHI, 2472+ 910, L) <br> | |
| − | Sub into pBca1256 (EcoRI/BamHI, 2472+ 910, L) | + | Product is pBca1256-K112602 {Flag} <br> |
| − | + | --------------------------------------------------- <br> | |
| − | Product is pBca1256-K112602 | + | Jw0019F Forward construction of Flag <br> |
| − | + | cgataGAATTCatgAGATCTgactacaaggatgacgacg <br> | |
| − | --------------------------------------------------- | + | Jw0020R Reverse construction of Flag <br> |
| − | + | ccagtGGATCCcttgtcgtcgtcatccttgtagtc<br> | |
| − | Jw0019F Forward construction of Flag | + | |
| − | + | ||
| − | cgataGAATTCatgAGATCTgactacaaggatgacgacg | + | |
| − | + | ||
| − | Jw0020R Reverse construction of Flag | + | |
| − | + | ||
| − | ccagtGGATCCcttgtcgtcgtcatccttgtagtc | + | |
| − | + | ||
| − | + | ||
===References=== | ===References=== | ||
| + | Terpe K. (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. 60:523-33. | ||
Latest revision as of 03:48, 10 May 2009
{˂FLAG˃}
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Later
Source
Wobble PCR Jw0019F / Jw0020R (45 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+ 910, L)
Product is pBca1256-K112602 {Flag}
Jw0019F Forward construction of Flag
cgataGAATTCatgAGATCTgactacaaggatgacgacg
Jw0020R Reverse construction of Flag
ccagtGGATCCcttgtcgtcgtcatccttgtagtc
References
Terpe K. (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. 60:523-33.