Difference between revisions of "Part:BBa K4083000:Design"

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<partinfo>BBa_K4083000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4083000 SequenceAndFeatures</partinfo>
  
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===Design Notes===
 
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https://static.igem.org/mediawiki/parts/a/a2/BBa_K4083000-RPGDuo2_plasmid.png
 
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Figure 1. Illustration of RPGDuo2 plasmid.
 
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As illustrated in Figure 1, this plasmid contains two multiple cloning sites (MCS 1 and MCS2) with two transcriptional terminators. The MCS1 and MCS2 are regulated by IPTG-inducible Ptac and aTc-inducible PtetR/tetA promoters respectively. Moreover, there are lacI and tetR dependent repression systems that are regulated by Ptac and PtetR/tetA respectively. They were integrated for metabolic engineering purposes. There is a kanamycin resistance gene (KanR). There are two origins of replication to allow replication in E. coli (colE1) and P. putida (pRO1600).
 
  
 
===Source===
 
===Source===

Latest revision as of 09:59, 20 October 2021


pRGPDuo2 plasmid for P. putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3853
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3880
    Illegal BamHI site found at 31
    Illegal XhoI site found at 2175
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6480
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

Modified from pRG_Duet for Corynebacterium glutamicum Gauttam et al.

References

[1] Gauttam, R., Mukhopadhyay, A., & Singer, S. W. (2020). Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida. Plasmid, 110. https://doi.org/10.1016/j.plasmid.2020.102514