Difference between revisions of "Part:BBa K3765003"

 
 
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<partinfo>BBa_K3765003 short</partinfo>
 
<partinfo>BBa_K3765003 short</partinfo>
  
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For purification of tagged proteins using Ni-NTA affinity chromatography.
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Decahistidine tag which can be used for purification of tagged proteins using Ni-NTA affinity chromatography. This tag can be placed either at the N- or the C-terminus of the protein. This part is an ''E. coli'' codon-optimized version of [https://parts.igem.org/Part:BBa_K823037 BBa_K823037].
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This part was used in our project for purification of the SpyCatcher002-dCBD and the OpdA-SpyTag002 constructs by Ni-NTA affinity column chromatography.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3765003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3765003 SequenceAndFeatures</partinfo>

Latest revision as of 05:52, 21 October 2021


10xHis Tag

Decahistidine tag which can be used for purification of tagged proteins using Ni-NTA affinity chromatography. This tag can be placed either at the N- or the C-terminus of the protein. This part is an E. coli codon-optimized version of BBa_K823037.

Usage and Biology

This part was used in our project for purification of the SpyCatcher002-dCBD and the OpdA-SpyTag002 constructs by Ni-NTA affinity column chromatography.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]