Difference between revisions of "Part:BBa J70026"

(Usage and Biology)
 
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===Usage and Biology===
 
===Usage and Biology===
  
MIC for E. coli DH10B cells is 4 ug/ml chloramphenicol.
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MIC for E. coli DH10B cells is 4 ug/ml chloramphenicol.  This is near background; the cassette appears not to function in E. coli. Horinouchi (1982) says this cassette is inducible.  Growth at 0.5 ug/ml for 30 minutes, followed by resistance at 20 ug/ml in E. coli.  See part J70304 for a version with the 5' UTR eliminated in an effort to eliminate this behavior.
  
 
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Latest revision as of 17:02, 6 May 2009

cat cassette (chloramphenicol resistance)

cat gene and promoter from pC194 plasmid, Genbank V01277.

Usage and Biology

MIC for E. coli DH10B cells is 4 ug/ml chloramphenicol. This is near background; the cassette appears not to function in E. coli. Horinouchi (1982) says this cassette is inducible. Growth at 0.5 ug/ml for 30 minutes, followed by resistance at 20 ug/ml in E. coli. See part J70304 for a version with the 5' UTR eliminated in an effort to eliminate this behavior.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]