Difference between revisions of "Part:BBa K3738009"
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MlrA, or microcystinase, cleaves the peptide backbone of MC-LR cyanotoxins produced by microcystis aeruginosa cyanobacteria. This crucial step results in the peptide losing the cyclic structure, and therefore the majority of its toxicity. Further degradation continues in an enzymatic pathway by interactions with other peptidolytic enzymes from the microcystinase gene cluster. This composite part includes the IPTG-inducible T7 promoter (BBa_J64997), RBS BBa_B0034, microcystinase coding region BBa_K3738019, and double terminator BBa_B0015. | MlrA, or microcystinase, cleaves the peptide backbone of MC-LR cyanotoxins produced by microcystis aeruginosa cyanobacteria. This crucial step results in the peptide losing the cyclic structure, and therefore the majority of its toxicity. Further degradation continues in an enzymatic pathway by interactions with other peptidolytic enzymes from the microcystinase gene cluster. This composite part includes the IPTG-inducible T7 promoter (BBa_J64997), RBS BBa_B0034, microcystinase coding region BBa_K3738019, and double terminator BBa_B0015. | ||
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| + | The Lethbridge 2022 Team added experience to this part as a part of their contributions for the Bronze Medal Requirement. | ||
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Latest revision as of 08:54, 12 October 2022
MlrA (Microcystinase) Composite Part
MlrA, or microcystinase, cleaves the peptide backbone of MC-LR cyanotoxins produced by microcystis aeruginosa cyanobacteria. This crucial step results in the peptide losing the cyclic structure, and therefore the majority of its toxicity. Further degradation continues in an enzymatic pathway by interactions with other peptidolytic enzymes from the microcystinase gene cluster. This composite part includes the IPTG-inducible T7 promoter (BBa_J64997), RBS BBa_B0034, microcystinase coding region BBa_K3738019, and double terminator BBa_B0015.
The Lethbridge 2022 Team added experience to this part as a part of their contributions for the Bronze Medal Requirement.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 556
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]