Difference between revisions of "Part:BBa K3771041"
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<partinfo>BBa_K3771041 short</partinfo> | <partinfo>BBa_K3771041 short</partinfo> | ||
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<br><b style="font-size:1.3rem">Description</b> | <br><b style="font-size:1.3rem">Description</b> | ||
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<br>L-cysteine dioxygenase (CDO1) is an enzyme that weighs 23 kDa. CDO1 functions in the L-cysteine sulfinic acid taurine biosynthesis pathway, converting L-cysteine to L-cysteine sulfinic acid. [1]<br> | <br>L-cysteine dioxygenase (CDO1) is an enzyme that weighs 23 kDa. CDO1 functions in the L-cysteine sulfinic acid taurine biosynthesis pathway, converting L-cysteine to L-cysteine sulfinic acid. [1]<br> | ||
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| + | <img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:50%;"> | ||
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| + | <p align="center">Fig. 1. Taurine pathways in <i>E. coli</i> [1,2].</p> | ||
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<br>CDO1 is part of the L-cysteine sulfinic acid pathway, one of three possible taurine synthesis pathways. CDO1 oxidizes L-cysteine naturally found in the intestine into L-cysteine sulfinic acid, which is later converted to taurine by CSAD [1].<br> | <br>CDO1 is part of the L-cysteine sulfinic acid pathway, one of three possible taurine synthesis pathways. CDO1 oxidizes L-cysteine naturally found in the intestine into L-cysteine sulfinic acid, which is later converted to taurine by CSAD [1].<br> | ||
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<br><b style="font-size:1.3rem">Usage | <br><b style="font-size:1.3rem">Usage | ||
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| − | <br>CDO1 was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC). | + | |
| + | <br>CDO1 was used in <i>in vivo</i> testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into <i>E. coli</i> to calculate taurine production using high-performance liquid chromatography (HPLC). | ||
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<br><b style="font-size:1.3rem">Characterization | <br><b style="font-size:1.3rem">Characterization | ||
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| + | <img src="https://2021.igem.org/wiki/images/7/72/T--NCKU_Tainan--cdo16his.jpg" style="width:50%;"> | ||
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| + | <p align="center">Fig. 2. PCR confirmation of <i>cdo1-6xHis</i></p> | ||
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| + | <br>CDO1 was used in <i>in vivo</i> testing of taurine production. The sequence for CDO1 enzyme and <i>trc</i> promoter were ligated and transformed into <i>E. coli</i> to calculate taurine production using high-performance liquid chromatography (HPLC). A 6xHis-tag is added to the C-terminal of the CDO1 protein, allowing for confirmation of CDO1 expression by western blot using the anti-6xHis antibody.<br> | ||
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<br><b style="font-size:1.3rem">References | <br><b style="font-size:1.3rem">References | ||
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| − | <br>Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093<br> | + | <br>1. Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093<br> |
| − | <!-- Add more about the biology of this part here | + | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 03:35, 22 October 2021
CDO1-6xHis
Description
L-cysteine dioxygenase (CDO1) is an enzyme that weighs 23 kDa. CDO1 functions in the L-cysteine sulfinic acid taurine biosynthesis pathway, converting L-cysteine to L-cysteine sulfinic acid. [1]
Fig. 1. Taurine pathways in E. coli [1,2].
CDO1 is part of the L-cysteine sulfinic acid pathway, one of three possible taurine synthesis pathways. CDO1 oxidizes L-cysteine naturally found in the intestine into L-cysteine sulfinic acid, which is later converted to taurine by CSAD [1].
Usage
CDO1 was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).
Characterization
Fig. 2. PCR confirmation of cdo1-6xHis
CDO1 was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC). A 6xHis-tag is added to the C-terminal of the CDO1 protein, allowing for confirmation of CDO1 expression by western blot using the anti-6xHis antibody.
References
1. Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 447
- 1000COMPATIBLE WITH RFC[1000]