Difference between revisions of "Part:BBa J70303"
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Assembly of pSCI2 plasmid origin and cat cassette into E. coli plasmid pSB1T3. | Assembly of pSCI2 plasmid origin and cat cassette into E. coli plasmid pSB1T3. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | MIC in E. coli DH10B cells is 1 ug/ml of chloramphenicol. This is near background; the cassette appears not to function in E. coli. | ||
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Latest revision as of 22:55, 5 May 2009
Test Cm resistance plasmid for Mesoplasma florum
Assembly of pSCI2 plasmid origin and cat cassette into E. coli plasmid pSB1T3.
Usage and Biology
MIC in E. coli DH10B cells is 1 ug/ml of chloramphenicol. This is near background; the cassette appears not to function in E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 372