Difference between revisions of "Part:BBa K3805209"
 
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<partinfo>BBa_K3805209 short</partinfo>
 
<partinfo>BBa_K3805209 short</partinfo>
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===(Toggle Switch)===
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This part is a Bi-stable switch(toggle switch).It is composed of trc promoter(BBa_K3805208))[https://parts.igem.org/Part:BBa_K3805208], P2 promoter(BBa_I746104))[https://parts.igem.org/Part:BBa_I746104], lacI- LVA (BBa_C0012))[https://parts.igem.org/Part:BBa_C0012]and ompT-mcherry(BBa_K3805886))[https://parts.igem.org/Part:BBa_K3805886].
  
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The bistable switch is composed of trc promoter(BBa_K3805208), P2 promoter(BBa_I746104), lacI- LVA (BBa_C0012)and mcherry-ompt(BBa_K3805886).When AIP is released, it will act on P2 promoter and start the expression of lacI-LVA and downstream mcherry-ompt. LacI-LVA inhibits trc promoter.When the concentration of AIP decreases, the P2 promoter will be closed, and the inhibition of lacI-LVA on the trc promoter will be relieved.The trc promoter starts the expression of the target protein.
 
  
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===Usage and Biology===
 
===Usage and Biology===
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At the presence of autoinducing peptides(AIPs), P2 promoter will be activated by phosphorylated AgrA (BBa_K38055))[https://parts.igem.org/Part:BBa_K3805525]and initiate the expression of downstrem lacI-LVA and ompT. LacI-LVA inhibits trc promoter.As the concentration of AIP decreases, the downstream transcription of P2 promoter will be down-regulated and the inhibition of lacI-LVA on the trc promoter will thus be lifted.The trc promoter then initiates the its downstream expression.
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[[File:Bistable_Switch.png|500px|thumb|center|Figure 1 Images under fluorescence microscope.(A)Before putting into AIPs, red fluorescence was observed.(B).After putting into AIP, red fluorescence disappeared,green fluorescence was observed.
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(fluorescence microscope 10x100)]]
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Latest revision as of 03:56, 22 October 2021


Bi-stable Switch

(Toggle Switch)

This part is a Bi-stable switch(toggle switch).It is composed of trc promoter(BBa_K3805208))[1], P2 promoter(BBa_I746104))[2], lacI- LVA (BBa_C0012))[3]and ompT-mcherry(BBa_K3805886))[4].


Usage and Biology

At the presence of autoinducing peptides(AIPs), P2 promoter will be activated by phosphorylated AgrA (BBa_K38055))[5]and initiate the expression of downstrem lacI-LVA and ompT. LacI-LVA inhibits trc promoter.As the concentration of AIP decreases, the downstream transcription of P2 promoter will be down-regulated and the inhibition of lacI-LVA on the trc promoter will thus be lifted.The trc promoter then initiates the its downstream expression.


Figure 1 Images under fluorescence microscope.(A)Before putting into AIPs, red fluorescence was observed.(B).After putting into AIP, red fluorescence disappeared,green fluorescence was observed. (fluorescence microscope 10x100)



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]