Difference between revisions of "Part:BBa K792002:Experience"

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<partinfo>BBa_K792002 AddReview 5</partinfo>
 
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<I>CWChew</I>
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<I>Team NUS Singapore 2021</I>
 
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Successfully used in <partinfo>BBa_K3927018</partinfo>, to secrete Human Beta Defensin 2. Characterization of part performance was also done using a GFP reporter.
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Team NUS_Singapore 2021 uccessfully expressed GFP using this part to quantify the performance. Characterization of part performance was also done using a GFP reporter. Check NUS Singapore 2021 wiki to view more information: https://2021.igem.org/Team:NUS_Singapore/Results
  
 
https://2021.igem.org/wiki/images/thumb/6/6e/T--NUS_Singapore--results_tab1_2.png/800px-T--NUS_Singapore--results_tab1_2.png
 
https://2021.igem.org/wiki/images/thumb/6/6e/T--NUS_Singapore--results_tab1_2.png/800px-T--NUS_Singapore--results_tab1_2.png

Latest revision as of 06:43, 18 October 2021

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K792002

User Reviews

UNIQ0581f179276077f4-partinfo-00000000-QINU

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manugimenez

Successfully used in BBa_K792010, to secrete a Trp-riched peptide. Check BBa_K792010 entry to get more information about the experience.

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Team NUS Singapore 2021

Team NUS_Singapore 2021 uccessfully expressed GFP using this part to quantify the performance. Characterization of part performance was also done using a GFP reporter. Check NUS Singapore 2021 wiki to view more information: https://2021.igem.org/Team:NUS_Singapore/Results

800px-T--NUS_Singapore--results_tab1_2.png

Figure 1: Image of the media supernatant, resuspended cells and blank control media under gel visualizer blue light, media and supernatant are clearly fluorescent compared to the control media.

800px-T--NUS_Singapore--results_tab1_1.png

Figure 2: mFa-GFP construct assembled to test the performance of this secretion tag.

800px-T--NUS_Singapore--results_tab1_3.png

Figure 3: GFP absorbance of the supernatant and cells of both induced and uninduced pGmFaGFP-H (BY4741), after normalization to blank media. Induced cells secreted approximately 42% of the GFP, while uninduced cells produced and secreted significantly less GFP than induced cells.


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UNIQ0581f179276077f4-partinfo-00000005-QINU