Difference between revisions of "Part:BBa K3952000:Design"
 
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A SpaI Restriction Site was found at 259. To adhere to RCF1000 assembly, a silent mutation was applied to the codon located within 285 and 287. The original codon was AGA and was altered to AGG.   
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A SpeI Restriction Site was found at 259. To adhere to RCF1000 assembly, a silent mutation was applied to the codon located within 257 and 263. The original codon was AGA and was altered to AGG.   
  
  

Latest revision as of 16:12, 18 October 2021


APOB-100 (Wild Type)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a positive control of the APOB gene allele that will be used within a Flappase genetic testing system.


A SpeI Restriction Site was found at 259. To adhere to RCF1000 assembly, a silent mutation was applied to the codon located within 257 and 263. The original codon was AGA and was altered to AGG.


Source

The source of the APOB-100 sequence is the genomic sequence from Bos taurus, apolipoprotein B genome. (GeneBank number: NC_037338.1)

References

https://pubmed.ncbi.nlm.nih.gov/26763170/