Difference between revisions of "Part:BBa K3745040:Design"
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[1] Dueber, J., Wu, G., Malmirchegini, G. et al. Synthetic protein scaffolds provide modular control over metabolic flux. Nat Biotechnol 27, 753–759 (2009). https://doi.org/10.1038/nbt.1557 | [1] Dueber, J., Wu, G., Malmirchegini, G. et al. Synthetic protein scaffolds provide modular control over metabolic flux. Nat Biotechnol 27, 753–759 (2009). https://doi.org/10.1038/nbt.1557 | ||
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| + | [2] Du, J., Zhang, A., Hao, J., & Wang, J. (2017). Biosynthesis of di-rhamnolipids and variations of congeners composition in genetically-engineered Escherichia coli. Biotechnology Letters, 39(7), 1041–1048. https://doi.org/10.1007/s10529-017-2333-2 | ||
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| + | [3] Gong ZJ, Peng YF, Zhang YT, et al. Construction and optimization of Escherichia coli for producing rhamnolipid biosurfactant. Chin J Biotech, 2015, 31(7): 1050–1062. | ||
Latest revision as of 03:27, 18 October 2021
RhlABC + tags + scaffold
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2281
Illegal BamHI site found at 6640
Illegal XhoI site found at 6649 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2149
Illegal NgoMIV site found at 2554
Illegal NgoMIV site found at 4123
Illegal NgoMIV site found at 5446
Illegal NgoMIV site found at 5761
Illegal NgoMIV site found at 6076
Illegal NgoMIV site found at 6391
Illegal AgeI site found at 3166 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5676
Illegal BsaI site found at 5991
Illegal BsaI site found at 6306
Illegal BsaI site found at 6621
Illegal SapI.rc site found at 4262
Illegal SapI.rc site found at 5305
Source
This study
References
[1] Dueber, J., Wu, G., Malmirchegini, G. et al. Synthetic protein scaffolds provide modular control over metabolic flux. Nat Biotechnol 27, 753–759 (2009). https://doi.org/10.1038/nbt.1557
[2] Du, J., Zhang, A., Hao, J., & Wang, J. (2017). Biosynthesis of di-rhamnolipids and variations of congeners composition in genetically-engineered Escherichia coli. Biotechnology Letters, 39(7), 1041–1048. https://doi.org/10.1007/s10529-017-2333-2
[3] Gong ZJ, Peng YF, Zhang YT, et al. Construction and optimization of Escherichia coli for producing rhamnolipid biosurfactant. Chin J Biotech, 2015, 31(7): 1050–1062.