Difference between revisions of "Part:BBa K3738004:Design"
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===Source=== | ===Source=== | ||
| − | + | This part comes from the MS2 Bacteriophage. | |
===References=== | ===References=== | ||
Meng, R., Jiang, M., Cui, Z. et al. Structural basis for the adsorption of a single-stranded RNA bacteriophage. Nat Commun 10, 3130 (2019). https://doi.org/10.1038/s41467-019-11126-8 | Meng, R., Jiang, M., Cui, Z. et al. Structural basis for the adsorption of a single-stranded RNA bacteriophage. Nat Commun 10, 3130 (2019). https://doi.org/10.1038/s41467-019-11126-8 | ||
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| + | https://www.uniprot.org/uniprot/P03610 | ||
Latest revision as of 02:42, 22 October 2021
MS2 Bacteriophage Maturation Protein A with C-Terminal Histidine Tag and TEV Cleavage Site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is a modified version of BBa_K3738000, to which an C-terminal histidine tag and TEV cleavage site have been added. As maturation protein A has not been well studied for use in phage-like particles, we have designed various versions of the part for testing purposes.
This part has been codon optimized for use in E.coli.
Source
This part comes from the MS2 Bacteriophage.
References
Meng, R., Jiang, M., Cui, Z. et al. Structural basis for the adsorption of a single-stranded RNA bacteriophage. Nat Commun 10, 3130 (2019). https://doi.org/10.1038/s41467-019-11126-8