Difference between revisions of "Part:BBa K3738005"

Latest revision as of 08:53, 12 October 2022

MS2 Bacteriophage Capsid Protein with N-Terminal Histidine Tag

The MS2 capsid protein comprises most of the capsid of the MS2 phage, as well as MS2 phage-like particles (PLPs). A single MS2 capsid requires 178 copies of the capsid protein, as one copy of maturation protein A (BBa_K3738000), which is critical in allowing the capsid to bind to the cell and deliver its cargo.

The design of this part was based off of part BBa_K2683012, which is an MS2 capsid protein fused to a polyarginine tag cell-penetrating peptide on the N-terminus. This part has been modified from the original so as to have the polyarginine tag replaced with a histidine tag, to allow for purification as a monomer for capsid assembly in vitro. This part has been codon-optimized for use in E.coli.

The Lethbridge 2022 Team added experience to this part as a part of their contributions for the Bronze Medal Requirement.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3738005 short</partinfo>
-The MS2 capsid protein comprises most of the capsid of the MS2 phage, as well as MS2 phage-like particles (PLPs). A single MS2 capsid requires 178 copies of the capsid protein, as one copy of maturation protein A (BBa_K3738000), which is critical in allowing the capsid to bind to the cell and deliver its cargo. The design of this part was based of part BBa_K2683012, which is an MS2 capsid protein fused to a polyarginine tag cell-penetrating peptide on the N-terminus.  This part has been modified from the original so as to have the polyarginine tag replaced with a histidine tag, to allow for purification as a monomer for capsid assembly in vitro.
+The MS2 capsid protein comprises most of the capsid of the MS2 phage, as well as MS2 phage-like particles (PLPs). A single MS2 capsid requires 178 copies of the capsid protein, as one copy of maturation protein A (BBa_K3738000), which is critical in allowing the capsid to bind to the cell and deliver its cargo.
+The design of this part was based off of part BBa_K2683012, which is an MS2 capsid protein fused to a polyarginine tag cell-penetrating peptide on the N-terminus.  This part has been modified from the original so as to have the polyarginine tag replaced with a histidine tag, to allow for purification as a monomer for capsid assembly in vitro. This part has been codon-optimized for use in E.coli.
+The Lethbridge 2022 Team added experience to this part as a part of their contributions for the Bronze Medal Requirement.
 <!-- Add more about the biology of this part here