Difference between revisions of "Part:BBa K3738004:Design"

Latest revision as of 02:42, 22 October 2021

MS2 Bacteriophage Maturation Protein A with C-Terminal Histidine Tag and TEV Cleavage Site

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This part is a modified version of BBa_K3738000, to which an C-terminal histidine tag and TEV cleavage site have been added. As maturation protein A has not been well studied for use in phage-like particles, we have designed various versions of the part for testing purposes.

This part has been codon optimized for use in E.coli.

Source

This part comes from the MS2 Bacteriophage.

References

Meng, R., Jiang, M., Cui, Z. et al. Structural basis for the adsorption of a single-stranded RNA bacteriophage. Nat Commun 10, 3130 (2019). https://doi.org/10.1038/s41467-019-11126-8

https://www.uniprot.org/uniprot/P03610

@@ Line 7: / Line 7: @@
 ===Design Notes===
+This part is a modified version of BBa_K3738000, to which an C-terminal histidine tag and TEV cleavage site have been added. As maturation protein A has not been well studied for use in phage-like particles, we have designed various versions of the part for testing purposes.
 This part has been codon optimized for use in E.coli.
 ===Source===
-https://www.uniprot.org/uniprot/P03610
+This part comes from the MS2 Bacteriophage.
 ===References===
 Meng, R., Jiang, M., Cui, Z. et al. Structural basis for the adsorption of a single-stranded RNA bacteriophage. Nat Commun 10, 3130 (2019). https://doi.org/10.1038/s41467-019-11126-8
+https://www.uniprot.org/uniprot/P03610