Difference between revisions of "Part:BBa K3886026"

 
 
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<partinfo>BBa_K3886026 short</partinfo>
 
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<p>This composite part is used to achieve L-arabinose-induced Chromoprotein eforRed expression. We tested this circuit in our Hidro system. To learn more about Hidro system, please visit  <a href="https://2021.igem.org/Team:NDNF_China/Proof_Of_Concept">NDNF_China Proof-Of-Concept</a>.</p>
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L-arabinose-inducible Chromoprotein eforRed expression circuits
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===Characterization===
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<p>To determine whether encapsulated bacteria in Hidro can respond to these external signal inputs, we encapsulated Hidro with the bacteria containing a genetic circuit that expresses chromoprotein (eforRed) in response to L-arabinose induction (Figure 1A). We then incubated the beads at 37 °C in the absence of L-arabinose or in the presence of 10mM L-arabinose. We found that encapsulated cells exposed to L-arabinose exhibited a pink colour compared with encapsulated cells not exposed to L-arabinose (Figure 1B). Thus, gene expression in Hidro can be exogenously controlled by chemical inducers. </p>
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===Usage and Biology===
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<img src="https://2021.igem.org/wiki/images/e/ea/T--NDNF_China--pocf26.png" alt="" width=600">
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<h6 style="text-align:center">Figure 1: (A) The schematic of a L-arabinose–controlled genetic switch to produce chromoprotein eforRed; (B) eforRed protein production in Hidro.</h6>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 17:45, 21 October 2021


L-arabinose-inducible Chromoprotein eforRed expression circuits

This composite part is used to achieve L-arabinose-induced Chromoprotein eforRed expression. We tested this circuit in our Hidro system. To learn more about Hidro system, please visit NDNF_China Proof-Of-Concept.

Characterization

To determine whether encapsulated bacteria in Hidro can respond to these external signal inputs, we encapsulated Hidro with the bacteria containing a genetic circuit that expresses chromoprotein (eforRed) in response to L-arabinose induction (Figure 1A). We then incubated the beads at 37 °C in the absence of L-arabinose or in the presence of 10mM L-arabinose. We found that encapsulated cells exposed to L-arabinose exhibited a pink colour compared with encapsulated cells not exposed to L-arabinose (Figure 1B). Thus, gene expression in Hidro can be exogenously controlled by chemical inducers.

Figure 1: (A) The schematic of a L-arabinose–controlled genetic switch to produce chromoprotein eforRed; (B) eforRed protein production in Hidro.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1474
    Illegal AgeI site found at 1049
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1031