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This Cas8 protein from Vibrio cholerae is part of the insertion of transposable elements by guide RNA–assisted targeting (INTEGRATE) system devised by Vo et al.(2020). The INTEGRATE system consists of CRISPR RNA-guided integrases for bacterial genome engineering. In this system, Cas8 is part of the TniQ-Cascade protein complex which is necessary for RNA-guided DNA integration.(Klompe et al., 2019)
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This Cas8 protein from Vibrio cholerae is part of the insertion of transposable elements by guide RNA–assisted targeting (INTEGRATE) system devised by Vo et al.(2020). The INTEGRATE system consists of CRISPR RNA-guided integrases for bacterial genome engineering (see <a href="https://parts.igem.org/Part:BBa_K3776022"> BBa_K3776022</a>). In this system, Cas8 is part of the TniQ-Cascade protein complex which is necessary for RNA-guided DNA integration.(Klompe et al., 2019)
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Latest revision as of 18:06, 17 October 2021
cas8
This Cas8 protein from Vibrio cholerae is part of the insertion of transposable elements by guide RNA–assisted targeting (INTEGRATE) system devised by Vo et al.(2020). The INTEGRATE system consists of CRISPR RNA-guided integrases for bacterial genome engineering (see BBa_K3776022). In this system, Cas8 is part of the TniQ-Cascade protein complex which is necessary for RNA-guided DNA integration.(Klompe et al., 2019)
Sequence and Features
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 217
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 434 Illegal BglII site found at 964
