Difference between revisions of "Part:BBa K3733032:Experience"
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===Applications of BBa_K3733032===
 
===Applications of BBa_K3733032===
 
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We transform <i>E.coli</i> DH5α strain with BBa_K3733032 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the reporter. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was spotted into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of thiosulfate were added Sodium sulfate solution. Add 2μL of sodium thiosulfate to each well for induction and measure the fluorescence with gain of 75 and OD<sub>600</sub> in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (<b>Figure 1</b>).  
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We transform <i>E.coli</i> DH5α strain with BBa_K3733032 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD<sub>600</sub> in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (<b>Figure 1</b>).  
 
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<center><img src="https://static.igem.org/mediawiki/parts/6/6f/T--HZAU-China--103-1.png" style="width:793px;height:360px"></center>
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<center><img src="https://static.igem.org/mediawiki/parts/6/6f/T--HZAU-China--103-1.png" style="width:784px;height:600px"></center>
 
<center><b>Figure 1.</b></b> OD<sub>600</sub> corrected RFU values culturing after 12 h after induction of different concentrations of sodium thiosulfate </center>
 
<center><b>Figure 1.</b></b> OD<sub>600</sub> corrected RFU values culturing after 12 h after induction of different concentrations of sodium thiosulfate </center>
 
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Latest revision as of 17:44, 17 October 2021


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Applications of BBa_K3733032

We transform E.coli DH5α strain with BBa_K3733032 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD600 in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (Figure 1).

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Figure 1. OD600 corrected RFU values culturing after 12 h after induction of different concentrations of sodium thiosulfate

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