Difference between revisions of "Part:BBa K3771011"

Latest revision as of 02:04, 22 October 2021

ompA promoter

Description

ompA promoter is a constitutive promoter that drives the expression of OmpA transmembrane protein in E. coli and other enterobacteria [1].

Biology

The ompA promoter is -35/-10 region upstream of the ompA gene in E. coli MG1655. OmpA protein is regulated by two overlapping promoters ompAp1 and ompAp2 [1,2].

Usage

ompA promoter was used to regulate the expression of OmpA/OprF chimeric protein in the IFN-γ sensing system.

Characterization

The ompA promoter sequence was amplified from E. coli MG1655 and cloned into our plasmid. Agarose gel electrophoresis results confirming the construction with the ompA promoter are shown in Fig. 1.

Fig. 1. Confirmation of P_ompA fragment by PCR. M: Marker; Lane 1: P_ompA (303 bp)

References

1. Cole ST, Bremer E, Hindennach I, Henning U. Characterisation of the promoters for the ompA gene which encodes a major outer membrane protein of Escherichia coli. Molecular and General Genetics MGG. 1982;188(3):472-479. doi:10.1007/bf00330051
2. Escherichia coli K-12 substr. MG1655 ompA. Biocyc.org. Published 2021. Accessed September 8, 2021.
3. Udekwu KI. Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Genes & Development. 2005;19(19):2355-2366. doi:10.1101/gad.354405

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 </b>
 <br>
-<br>ompA promoter is a constitutive promoter that drives the expression of OmpA transmembrane protein in E. coli and other enterobacteria [1].
+<br><i>ompA</i> promoter is a constitutive promoter that drives the expression of OmpA transmembrane protein in <i>E. coli</i> and other enterobacteria [1].
 <br><br><b style="font-size:1.3rem">Biology
 </b>
 <br>
-<br>The ompA promoter is -35/-10 region upstream of the OmpA gene in E. coli MG1655. OmpA protein is regulated by two overlapping promoters ompAp1 and ompAp2 [1,2].
+<br>The <i>ompA</i> promoter is -35/-10 region upstream of the <i>ompA</i> gene in <i>E. coli</i> MG1655. OmpA protein is regulated by two overlapping promoters <i>ompAp1</i> and <i>ompAp2</i> [1,2].
 <br><br><b style="font-size:1.3rem">Usage
 </b>
-<br><br>ompA promoter was used to regulate the expression of OmpA/OprF chimeric protein in the IFN-γ sensing system.
+<br><br><i>ompA</i> promoter was used to regulate the expression of OmpA/OprF chimeric protein in the IFN-γ sensing system.
+<br><br><b style="font-size:1.3rem">Characterization
+</b>
+<br><br>The <i>ompA</i> promoter sequence was amplified from <i>E. coli</i> MG1655 and cloned into our plasmid. Agarose gel electrophoresis results confirming the construction with the <i>ompA</i> promoter are shown in Fig. 1.
+<html>
+<br>
+<div style="width=100%; display:flex; align-items: center; justify-content: center;">
+<img src="https://2021.igem.org/wiki/images/b/b0/T--NCKU_Tainan--pcr_ompA_promoter.jpg" style="width:40%;">
+</div>
+  <p align="center">Fig. 1. Confirmation of <i>P<sub>ompA</sub></i> fragment by PCR. M: Marker; Lane 1: <i>P<sub>ompA</sub></i> (303 bp)</p>
+</html>
+<br><br><b style="font-size:1.3rem">References
+</b>
+<br>
+<br>1. Cole ST, Bremer E, Hindennach I, Henning U. Characterisation of the promoters for the ompA gene which encodes a major outer membrane protein of Escherichia coli. Molecular and General Genetics MGG. 1982;188(3):472-479. doi:10.1007/bf00330051
+<br>2. Escherichia coli K-12 substr. MG1655 ompA. Biocyc.org. Published 2021. Accessed September 8, 2021.
+<br>3. Udekwu KI. Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Genes & Development. 2005;19(19):2355-2366. doi:10.1101/gad.354405