Difference between revisions of "Part:BBa K3729001"
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Butyryl- CoA dehydrogenase catalyzes the rate-limiting step (Crotonyl-CoA to butyryl-CoA) of the acetyl-CoA butyrate synthesis pathway, further increasing the efficiency of bacteria’s butyrate production. We obtained the DNA sequence (BCD) encoded for butyryl- CoA dehydrogenase from the genome of Clostridium acetobutylicum. | Butyryl- CoA dehydrogenase catalyzes the rate-limiting step (Crotonyl-CoA to butyryl-CoA) of the acetyl-CoA butyrate synthesis pathway, further increasing the efficiency of bacteria’s butyrate production. We obtained the DNA sequence (BCD) encoded for butyryl- CoA dehydrogenase from the genome of Clostridium acetobutylicum. | ||
| − | < | + | <b>Construct design:</b>We optimized the DNA sequence for expression in E. coli and flanked the open reading frame with an upstream strong promoter, a strong ribosome binding site (RBS), and a double terminator. This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of butyryl-CoA dehydrogenase. Our composite part is synthesized by IDT. |
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| + | <img src="https://static.igem.org/mediawiki/parts/c/c5/T--KCIS_New_Taipei--BCD2.png" /> | ||
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Latest revision as of 03:26, 22 October 2021
BCD 2: (Butyryl- CoA Dehydrogenase)
Butyryl- CoA dehydrogenase catalyzes the rate-limiting step (Crotonyl-CoA to butyryl-CoA) of the acetyl-CoA butyrate synthesis pathway, further increasing the efficiency of bacteria’s butyrate production. We obtained the DNA sequence (BCD) encoded for butyryl- CoA dehydrogenase from the genome of Clostridium acetobutylicum.
Construct design:We optimized the DNA sequence for expression in E. coli and flanked the open reading frame with an upstream strong promoter, a strong ribosome binding site (RBS), and a double terminator. This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of butyryl-CoA dehydrogenase. Our composite part is synthesized by IDT.
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