Difference between revisions of "Part:BBa J70301:Design"
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primers: | primers: | ||
| − | caaataacggataattataaaataaaac | + | |
| + | |||
| + | GTTTCTTC GAATTC GCGGCCGC T TCTAGA G caaataacggataattataaaataaaac,J70301-F | ||
| + | |||
| + | GTTTCTTC CTGCAG CGGCCGC T ACTAGT A ctatgtggttaaatatatattatcatattg,J70301-R | ||
| + | |||
| + | |||
| + | 474647 - 474537 (reverse orientation) from NC_006055 | ||
===Source=== | ===Source=== | ||
Latest revision as of 20:39, 6 April 2009
Heat shock inducible promoter for Me. florum
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Low gc content, difficult PCR primers
primers:
GTTTCTTC GAATTC GCGGCCGC T TCTAGA G caaataacggataattataaaataaaac,J70301-F
GTTTCTTC CTGCAG CGGCCGC T ACTAGT A ctatgtggttaaatatatattatcatattg,J70301-R
474647 - 474537 (reverse orientation) from NC_006055
Source
PCR from lon protease promoter of NC_006055.