Difference between revisions of "Part:BBa J70300:Design"

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===Design Notes===
 
===Design Notes===
−
XbaI site removed from the upstream region, by t->c mutation at 16.
+
XbaI site removed from the upstream region, by t->c mutation at 16 inserted into the F primer.
  
  
−
cagttaaaaacattcaagataagtttgc
+
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGcagttaaaaacattccagataagtttgc,J70300-F
  
−
attaaattcaaaatatcttctgcttc
+
GTTTCTTCCTGCAGCGGCCGCTACTAGTAattaaattcaaaatatcttctgcttc,J70300-R
  
 
===Source===
 
===Source===

Latest revision as of 20:37, 6 April 2009

Strong Me. florum promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

XbaI site removed from the upstream region, by t->c mutation at 16 inserted into the F primer.


GTTTCTTCGAATTCGCGGCCGCTTCTAGAGcagttaaaaacattccagataagtttgc,J70300-F

GTTTCTTCCTGCAGCGGCCGCTACTAGTAattaaattcaaaatatcttctgcttc,J70300-R

Source

53556 53678 approximately from NC_006055


References