Difference between revisions of "Part:BBa J70300:Design"
(→Design Notes) |
(→Design Notes) |
||
| (4 intermediate revisions by the same user not shown) | |||
| Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | XbaI site removed from the upstream region, by t-> | + | XbaI site removed from the upstream region, by t->c mutation at 16 inserted into the F primer. |
| − | + | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGcagttaaaaacattccagataagtttgc,J70300-F | |
| + | |||
| + | GTTTCTTCCTGCAGCGGCCGCTACTAGTAattaaattcaaaatatcttctgcttc,J70300-R | ||
===Source=== | ===Source=== | ||
Latest revision as of 20:37, 6 April 2009
Strong Me. florum promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
XbaI site removed from the upstream region, by t->c mutation at 16 inserted into the F primer.
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGcagttaaaaacattccagataagtttgc,J70300-F
GTTTCTTCCTGCAGCGGCCGCTACTAGTAattaaattcaaaatatcttctgcttc,J70300-R
Source
53556 53678 approximately from NC_006055