Difference between revisions of "Part:BBa K3930008"
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<h2>Introduction</h2> | <h2>Introduction</h2> | ||
− | <p>This sequence codes for the linalool synthase, which | + | <p>This sequence codes for the linalool synthase, which transforms GPP into linalool. The sequence derives from the <i>Lavandula angustifolia</i> genome. We take advantage of the publication of (Amiri et al., 2016). |
<p>There is already <a href="https://parts.igem.org/Part:BBa_K3052002" class="pr-0" target="_blank">(BBa_K3052002)</a> on the registry for linalool synthase. This part is codon optimized for translation in <i>E. coli</i>, but our is codon optimized for translation in <i>S. cerevisiae</i>.</p> | <p>There is already <a href="https://parts.igem.org/Part:BBa_K3052002" class="pr-0" target="_blank">(BBa_K3052002)</a> on the registry for linalool synthase. This part is codon optimized for translation in <i>E. coli</i>, but our is codon optimized for translation in <i>S. cerevisiae</i>.</p> | ||
<h2>Testing</h2> | <h2>Testing</h2> |
Latest revision as of 09:04, 17 October 2021
Gene coding for the Linalool synthase
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 1000COMPATIBLE WITH RFC[1000]
Introduction
This sequence codes for the linalool synthase, which transforms GPP into linalool. The sequence derives from the Lavandula angustifolia genome. We take advantage of the publication of (Amiri et al., 2016).
There is already (BBa_K3052002) on the registry for linalool synthase. This part is codon optimized for translation in E. coli, but our is codon optimized for translation in S. cerevisiae.
Testing
This part is one of the component of the pFLEUR part (BBa_K3930000) and has been succesfully cloned into the LycoYeast S. cerevisiae strain genome. The Toulouse team could not test this part for reasons of time, and preferred to focus on the part characterizations that allow the production of α and β-ionone for the floral aspect of the violet perfume.
References
- Amiri P, Shahpiri A, Asadollahi MA, Momenbeik F, Partow S. 2016. Metabolic engineering of Saccharomyces cerevisiae for linalool production. Biotechnol Lett. 38(3):503–508. doi:10.1007/s10529-015-2000-4.