Difference between revisions of "Part:BBa K3868105"

 
 
Line 5: Line 5:
 
pET-24a-SMAP-29 plasmid contains pT7, lacO, SMAP-29, thrombin, eGFP-His-tag etc.Based on the plasmid of pET-24a, the C-terminal of SMAP-29 was fused with eGFP in order to characterize the yield of SMAP-29. Moreover, in order to purify the SMAP-29, the enzyme loci of thrombin was inserted between SMAP-29 and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-SMAP-29-eGFP. The fluorescence intensity of eGFP could be used to indicate the expression level of SMAP-29.
 
pET-24a-SMAP-29 plasmid contains pT7, lacO, SMAP-29, thrombin, eGFP-His-tag etc.Based on the plasmid of pET-24a, the C-terminal of SMAP-29 was fused with eGFP in order to characterize the yield of SMAP-29. Moreover, in order to purify the SMAP-29, the enzyme loci of thrombin was inserted between SMAP-29 and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-SMAP-29-eGFP. The fluorescence intensity of eGFP could be used to indicate the expression level of SMAP-29.
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
−
 
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3868105 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3868105 SequenceAndFeatures</partinfo>
  
  
 +
 +
===Usage and Biology===
 +
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Based on the plasmid of pET-24a. The C-terminal of Antibacterial peptides(AMP) was fused with eGFP in order to characterize the yield of AMP. The pET-24a was provided by our PI. Moreover, in order to purify the AMP, the enzyme loci of thrombin was inserted between AMP and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-AMP-eGFP. As a result, the pET-AMP-eGFP plasmids could not only indicate the expression level of AMPs by the fluorescence intensity of eGFP, but also be good for later purification and separation with His-Tag and the enzyme loci of thrombin. (Fig. 1)
 +
 +
<html>
 +
<div align="center">
 +
    <figure>
 +
        <img src="https://2021.igem.org/wiki/images/c/c0/T--NNU-China--part-1.png" width="30%" style="float:center">
 +
        <figcaption>
 +
        <p style="font-size:1rem">
 +
        </p>
 +
        </figcaption>
 +
    </figure>
 +
</div>
 +
</html>
 +
<div align="center">
 +
:'''Fig 1. The Schematic diagram of pET-AMP-eGFP '''
 +
</div>
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3868105 parameters</partinfo>
 
<partinfo>BBa_K3868105 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 11:39, 19 October 2021


pET-SMAP-29-eGFP

pET-24a-SMAP-29 plasmid contains pT7, lacO, SMAP-29, thrombin, eGFP-His-tag etc.Based on the plasmid of pET-24a, the C-terminal of SMAP-29 was fused with eGFP in order to characterize the yield of SMAP-29. Moreover, in order to purify the SMAP-29, the enzyme loci of thrombin was inserted between SMAP-29 and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-SMAP-29-eGFP. The fluorescence intensity of eGFP could be used to indicate the expression level of SMAP-29.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

        Based on the plasmid of pET-24a. The C-terminal of Antibacterial peptides(AMP) was fused with eGFP in order to characterize the yield of AMP. The pET-24a was provided by our PI. Moreover, in order to purify the AMP, the enzyme loci of thrombin was inserted between AMP and eGFP, and the label of 6*His was inserted at the end of eGFP, yielding the plasmid of pET-AMP-eGFP. As a result, the pET-AMP-eGFP plasmids could not only indicate the expression level of AMPs by the fluorescence intensity of eGFP, but also be good for later purification and separation with His-Tag and the enzyme loci of thrombin. (Fig. 1)

Fig 1. The Schematic diagram of pET-AMP-eGFP