Difference between revisions of "Part:BBa K3753003"
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| − | [[File:Gold.png|width='100%' valign='top'| |center|thumb| | + | [[File:Gold.png|width='100%' valign='top'| |center|thumb|480*500px|''<b>Fig.1</b> The fluorescence intensity of GFP expressed by engineered promoters]] |
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| − | When assembled with | + | When assembled with GFP <partinfo>BBa_K3112009</partinfo>, and CYC1 terminator <partinfo>BBa_K3384311</partinfo> in pRS426, pTEF2 regulated the expression of GFP. |
| − | Three pTEF2-based synthetic hybrid promoters were designed to enhance promoter activity. For detailed information, please visit | + | Three pTEF2-based synthetic hybrid promoters were designed to enhance promoter activity. For detailed information, please visit UAS<sub>CLB</sub>-pTEF <partinfo>BBa_K3753011</partinfo>, UAS<sub>CIT</sub>-pTEF <partinfo>BBa_K3753012</partinfo>, UAS<sub>TEF</sub>-pTEF <partinfo>BBa_K3753013</partinfo>. |
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Latest revision as of 16:10, 16 October 2021
pTEF2
pTEF2 is a strong constitutive promoter in Saccharomyces cerevisiae, which controls the transcription of its downstream sequence coding for translational elongation factor EF-1 α.
Characterization
When assembled with GFP BBa_K3112009, and CYC1 terminator BBa_K3384311 in pRS426, pTEF2 regulated the expression of GFP.
Three pTEF2-based synthetic hybrid promoters were designed to enhance promoter activity. For detailed information, please visit UASCLB-pTEF BBa_K3753011, UASCIT-pTEF BBa_K3753012, UASTEF-pTEF BBa_K3753013.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 490
- 1000COMPATIBLE WITH RFC[1000]