Difference between revisions of "Part:BBa K3753013"
 
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UAS<sub>TEF</sub>-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from translational elongation factor EF-1 &alpha; <em>tef1</em>, and the core promoter <em>tef2</em>. The tandem UAS elements of hybrid promoter could serve as synthetic transcriptional amplifiers to control expression levels.
 
UAS<sub>TEF</sub>-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from translational elongation factor EF-1 &alpha; <em>tef1</em>, and the core promoter <em>tef2</em>. The tandem UAS elements of hybrid promoter could serve as synthetic transcriptional amplifiers to control expression levels.
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===Characterization===
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[[File:Gold.png|width='100%' valign='top'| |center|thumb|480*500px|''<b>Fig.1</b> The fluorescence intensity of GFP expressed by engineered promoters]]
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When assembled with GFP <partinfo>BBa_K3112009</partinfo>, and <partinfo>BBa_K3384311</partinfo> in pRS426, this construct expressed GFP. Then the activity of the bybrid promoter can be quantitatively measured through flow cytometer. As is shown in <b>Fig. 1</b>, the UAS<sub>TEF</sub>-pTEF promoter strength was expanded beyond the pTEF by 1.2-fold.
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Latest revision as of 16:11, 16 October 2021


UASTEF-pTEF

UASTEF-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from translational elongation factor EF-1 α tef1, and the core promoter tef2. The tandem UAS elements of hybrid promoter could serve as synthetic transcriptional amplifiers to control expression levels.

Characterization


Fig.1 The fluorescence intensity of GFP expressed by engineered promoters


When assembled with GFP BBa_K3112009, and BBa_K3384311 in pRS426, this construct expressed GFP. Then the activity of the bybrid promoter can be quantitatively measured through flow cytometer. As is shown in Fig. 1, the UASTEF-pTEF promoter strength was expanded beyond the pTEF by 1.2-fold.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 629
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 629
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 629
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 629
    Illegal NgoMIV site found at 1151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 167
    Illegal BsaI.rc site found at 378
    Illegal BsaI.rc site found at 587