Difference between revisions of "Part:BBa K3893027"

Latest revision as of 14:14, 16 October 2021

AHL induced expression of Lysis protein.

Figure 1. SBOL Visual representation of the part

AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part, together with K3893026, is a component of the intermediate part K3893029 to assemble the release system device (K3893030) of our Agrobactory 593. It is used to express the lysis protein phiX174 when AHL (bound to to LuxR) is present, a fundamental piece of our population oscillator. release device.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

BBa_K2656003: the R0062 inducible pLux promoter in its Golden Braid compatible version
BBa_K2656010: the B0032 weak ribosome biding site in its Golden Braid compatible version
BBa_K2656015: the phiX174 lysis protein
Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Part BBa_K2656015 is the lysis gene from the enterobacteria phage phiX174 coding sequence. The lysis gene E of coliphage PhiX174 encodes a 91-amino acid (aa) residue protein of approximately 10 kDa that mediates inhibition of peptidoglycan biosynthesis of Gram-negative bacteria membrane.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3893027 short</partinfo>
-[[File:T--Ecuador--K3893027_sbolv.png|200px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the part ]]
+[[File:T--Ecuador--K3893027_sbolv.png|300px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the part ]]
 AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.
-This composite part, together with [https://parts.igem.org/Part:BBa_K3893026 K3893026], is a component of the intermediate part [https://parts.igem.org/Part:BBa_K3893028]  to assemble the release system device ([https://parts.igem.org/Part:BBa_K3893030]) of our Agrobactory 593. It is used to express the lysis protein Phi174 when AHL (bound to to LuxR) is present, a fundamental piece of our population oscillator. release device.
+This composite part, together with [https://parts.igem.org/Part:BBa_K3893026 K3893026], is a component of the intermediate part [https://parts.igem.org/Part:BBa_K3893029 K3893029]  to assemble the release system device ([https://parts.igem.org/Part:BBa_K3893030 K3893030]) of our Agrobactory 593. It is used to express the lysis protein phiX174 when AHL (bound to to LuxR) is present, a fundamental piece of our population oscillator. release device.
 This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
@@ Line 18: / Line 18: @@
 <li></html>[https://parts.igem.org/Part:BBa_K2656010 BBa_K2656010]: the [https://parts.igem.org/Part:BBa_B0032 B0032] weak ribosome biding site in its Golden Braid compatible version <html></li>
-<li></html> [https://parts.igem.org/Part:BBa_K2656015 BBa_K2656015]: the Pho174 lysis protein <html></li>
+<li></html> [https://parts.igem.org/Part:BBa_K2656015 BBa_K2656015]: the phiX174 lysis protein <html></li>
 <li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>