Difference between revisions of "Part:BBa K3753011:Design"

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===Design Notes===
 
===Design Notes===
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UAS<sub>CLB</sub>-pTEF is synthesized by fusing three UAS<sub>CLB</sub> tandem to the <em>tef2</em> promoter (BBa_K3753003). UAS<sub>CLB</sub> was obtained by PCR amplification using <em>Saccharomyces cerevisiae</em> BY4741 genome as template. The amplified product is a 240bp sequence upstream of the <em>clb2</em> promoter start codon.
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UAS<sub>CLB</sub>-pTEF is synthesized by fusing three UAS<sub>CLB</sub> tandem to the <em>tef2</em> promoter (<partinfo>BBa_K3753003</partinfo>). UAS<sub>CLB</sub> was obtained by PCR amplification using <em>Saccharomyces cerevisiae</em> BY4741 genome as template. The amplified product is a 240bp sequence upstream of the <em>clb2</em> promoter start codon.
  
 
===Source===
 
===Source===
  
−
Saccharomyces cerevisiae
+
<em>Saccharomyces cerevisiae</em>
  
 
===References===
 
===References===
 +
John Blazeck et al. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters[J]. Biotechnology and Bioengineering, 2012, 109(11) : 2884-2895.
 +
 +
Van Slyke Ceri and Grayhack Elizabeth J.. The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region[J]. 2003, 31(15) : 4597-4607.

Latest revision as of 16:19, 16 October 2021


UASCLB-pTEF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 747
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 747
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 747
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 747
    Illegal NgoMIV site found at 1269
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

UASCLB-pTEF is synthesized by fusing three UASCLB tandem to the tef2 promoter (BBa_K3753003). UASCLB was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The amplified product is a 240bp sequence upstream of the clb2 promoter start codon.

Source

Saccharomyces cerevisiae

References

John Blazeck et al. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters[J]. Biotechnology and Bioengineering, 2012, 109(11) : 2884-2895.

Van Slyke Ceri and Grayhack Elizabeth J.. The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region[J]. 2003, 31(15) : 4597-4607.