Difference between revisions of "Part:BBa K3866038"

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The coding sequence was domesticated. We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo.
 
The coding sequence was domesticated. We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo.
  
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[[Image:T--Thessaly--J23115-sfGFP-term.png|800px|thumb|none|<I><b>Figure 1.</b> The first level α module of the Propionate Production: α1:ParaBAD:RBS-smb-Double terminator </i>]]
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[[Image:T--Thessaly--J23115-sfGFP-term.png|800px|thumb|none|<I><b>Figure 1.</b> The level α module of the sfGFP Translational Unit: α1: J23115:RBS-sfGFP-Double terminator</i>]]
  
 
===Verification of Cloning===
 
===Verification of Cloning===
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[[File:T--Thessaly--gfpgel.png|800px|thumb|none|<i><b>Figure 2.</b>: (C=Cut, U=Uncut) Restriction digestion of α1-sbm with: EcoRI, Expected bands: 6345bp, 2947bp, 597bp, Positive result: C2</i>]]
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[[File:T--Thessaly--gfpgel.png|800px|thumb|none|<i><b>Figure 2.</b>: (C=Cut, U=Uncut) Restriction digestion of α-sfGFP TU (samples 3,4,5) with: HindIII, Expected bands: 2847bp, 801bp, 146bp, Positive result: C5</i>]]
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===Experimental Use and Experience===
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This part showed functionality at the following parts: <bbpart>BBa_K3866029</bbpart>, <bbpart>BBa_K3866031</bbpart>
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===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K3866038 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3866038 SequenceAndFeatures</partinfo>

Latest revision as of 12:13, 17 October 2021


J23115:sfGFP:Terminator

Usage and Biology

This TU includes the sfGFP gene placed under the control of the constitutive Anderson promoter J23115 BBa_J23115.

Design Notes

The coding sequence was domesticated. We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo.

Figure 1. The level α module of the sfGFP Translational Unit: α1: J23115:RBS-sfGFP-Double terminator

Verification of Cloning

Figure 2.: (C=Cut, U=Uncut) Restriction digestion of α-sfGFP TU (samples 3,4,5) with: HindIII, Expected bands: 2847bp, 801bp, 146bp, Positive result: C5

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 192
  • 1000
    COMPATIBLE WITH RFC[1000]