Difference between revisions of "Part:BBa K3962350"
 
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<partinfo>BBa_K3962350 short</partinfo>
 
<partinfo>BBa_K3962350 short</partinfo>
  
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Sok is the antitoxin of the Hok/Sok toxin-antitoxin system. The Hok/Sok is a type I toxin-antitoxin system originating from the R1 plasmid in E. coli. The Sok transcript can bind to a homologous region onHok mRNA and prevent toxin expression. When E. coli undergoes cell division, the two daughter cells inherit the long-lived Hok toxin from the parent cell. Due to the short half-life of the Sok antitoxin, daughter cells inherit only small amounts and it is quickly degraded.
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HOK is the toxin of the HOK/SOK toxin-antitoxin system. The HOK/SOK is a type I toxin-antitoxin system originating from the R1 plasmid in E. coli. The SOK transcript can bind to a homologous region on HOK mRNA and prevent toxin expression. When E. coli undergoes cell division, the two daughter cells inherit the long-lived HOK toxin from the parent cell. Due to the short half-life of the SOK antitoxin, daughter cells inherit only small amounts and it is quickly degraded.  
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The gel electrophoresis of pSB1A3-HOK is shown in Figure 1. The result in lane 18 shows that HOK is present in the biobrick.
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https://2021.igem.org/wiki/images/6/6d/T--Leiden--Parts-HOK.png
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'''''Figure 1''''' ''The gel result of pSB1A3-HOK (lane 18)''
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:40, 21 October 2021


HOK - Toxin in Hok/Sok

HOK is the toxin of the HOK/SOK toxin-antitoxin system. The HOK/SOK is a type I toxin-antitoxin system originating from the R1 plasmid in E. coli. The SOK transcript can bind to a homologous region on HOK mRNA and prevent toxin expression. When E. coli undergoes cell division, the two daughter cells inherit the long-lived HOK toxin from the parent cell. Due to the short half-life of the SOK antitoxin, daughter cells inherit only small amounts and it is quickly degraded.

The gel electrophoresis of pSB1A3-HOK is shown in Figure 1. The result in lane 18 shows that HOK is present in the biobrick.

T--Leiden--Parts-HOK.png

Figure 1 The gel result of pSB1A3-HOK (lane 18)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]