Difference between revisions of "Part:BBa K4047004"
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===Long Description=== | ===Long Description=== | ||
− | This is the reverse primer | + | This is the reverse primer that pairs with the forward primer BBa_K4047005. Together they amplify and allow insertion of transaldolase and fructose-1,6-bisphosphatase into pGEM-tal+ fbp (BBa_K4047038). The overlap sequence allows proper Gibson assembly with the pGEM-tal+fbp backbone (BBa_K4047024). The annealing portion (all caps) allows annealing to the wild type Synechococcus elongatus PCC 7942 genome for proper amplification. |
This primer does not contain any BioBrick incompatible sequences, making it a very useful option for teams that want to amplify transaldolase from the wild type genome. | This primer does not contain any BioBrick incompatible sequences, making it a very useful option for teams that want to amplify transaldolase from the wild type genome. |
Latest revision as of 04:28, 16 October 2021
primerTF_tal-reverse
Rev primer for transadolase
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Long Description
This is the reverse primer that pairs with the forward primer BBa_K4047005. Together they amplify and allow insertion of transaldolase and fructose-1,6-bisphosphatase into pGEM-tal+ fbp (BBa_K4047038). The overlap sequence allows proper Gibson assembly with the pGEM-tal+fbp backbone (BBa_K4047024). The annealing portion (all caps) allows annealing to the wild type Synechococcus elongatus PCC 7942 genome for proper amplification.
This primer does not contain any BioBrick incompatible sequences, making it a very useful option for teams that want to amplify transaldolase from the wild type genome.