Difference between revisions of "Part:BBa K3753003"
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<partinfo>BBa_K3753003 short</partinfo>
 
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p<em>TEF2</em> is a strong constitutive promoter in <em>Saccharomyces cerevisiae</em>. The translation elongation factor EF-1 alpha of the yeast Saccharomyces cerevisiae is coded for by two genes, called TEF1 and TEF2. TEF2 alone is sufficient to promote growth of the cells as shown with a strain deleted for TEF1.
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p<em>TEF2</em> is a strong constitutive promoter in <em>Saccharomyces cerevisiae</em>, which controls the transcription of its downstream sequence coding for translational elongation factor EF-1 &alpha;.
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===Characterization===
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<p> </p>
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[[File:Gold.png|width='100%' valign='top'| |center|thumb|480*500px|''<b>Fig.1</b> The fluorescence intensity of GFP expressed by engineered promoters]]
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<br>
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When assembled with GFP <partinfo>BBa_K3112009</partinfo>, and CYC1 terminator <partinfo>BBa_K3384311</partinfo> in pRS426, pTEF2 regulated the expression of GFP.  
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Three pTEF2-based synthetic hybrid promoters were designed to enhance promoter activity. For detailed information, please visit UAS<sub>CLB</sub>-pTEF <partinfo>BBa_K3753011</partinfo>, UAS<sub>CIT</sub>-pTEF <partinfo>BBa_K3753012</partinfo>, UAS<sub>TEF</sub>-pTEF <partinfo>BBa_K3753013</partinfo>.
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<!-- Add more about the biology of this part here
 
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Latest revision as of 16:10, 16 October 2021

pTEF2

pTEF2 is a strong constitutive promoter in Saccharomyces cerevisiae, which controls the transcription of its downstream sequence coding for translational elongation factor EF-1 α.

Characterization


Fig.1 The fluorescence intensity of GFP expressed by engineered promoters


When assembled with GFP BBa_K3112009, and CYC1 terminator BBa_K3384311 in pRS426, pTEF2 regulated the expression of GFP. Three pTEF2-based synthetic hybrid promoters were designed to enhance promoter activity. For detailed information, please visit UASCLB-pTEF BBa_K3753011, UASCIT-pTEF BBa_K3753012, UASTEF-pTEF BBa_K3753013.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 490
  • 1000
    COMPATIBLE WITH RFC[1000]