Difference between revisions of "Part:BBa K3788002"
 
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<p> BBa_K3788002 sequence is coding for P20 a chaperone protein in Bacillus thuringiensis.
 
<p> BBa_K3788002 sequence is coding for P20 a chaperone protein in Bacillus thuringiensis.
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This chaperon have a 6x Histine tag in N-ter to allow purification of the protein P20and specific detection by Western blot. The gene p20 was optimised for E. coli K12. </p>
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This chaperon has a 6x Histine tag in N-ter to allow purification of the protein P20and specific detection by Western blot. The gene p20 was optimised for E. coli K12. </p>
 
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Latest revision as of 09:06, 21 October 2021


6His-P20


BBa_K3788002 sequence is coding for P20 a chaperone protein in Bacillus thuringiensis. This chaperon has a 6x Histine tag in N-ter to allow purification of the protein P20and specific detection by Western blot. The gene p20 was optimised for E. coli K12.

Expected size :

6his_p20 gene is 570bp

6His_P20 protein is 20 kDa


P20 chaperon:

Was discovered in B.thuringiensis, a p20 gene in operon with cry11Aa and <i<cyt1Aa</i>. Thus, the P20 protein was shown to be necessary for the formation of inclusion bodies of Cry11Aa and Cyt1Aa. P20 would allow a greater production of these toxins and allow their stability (Cyt1Aa would be protected from the proteolytic action before its maturation). The co-production of P20 and Cyt1Aa in E.coli would limit its toxic effects in the producer cell.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 154
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]