Difference between revisions of "Part:BBa K3788001:Experience"
 
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<h2> PCR amplification of flag<i>Cry11Aa</i> gene : </h2>
 
<h2> PCR amplification of flag<i>Cry11Aa</i> gene : </h2>
  
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<p> The DNA sequence of flag<i>cry11Aa</i> was amplify by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is <b>1956bp </b> and a 2000bp band is observed. Amplification of DNAs sequences with primers were validated.</p>
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<p> The DNA sequence of flag<i>cry11Aa</i> was amplified by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is <b>1956bp </b> and a 2000bp band is observed. Amplification of DNAs sequences with primers was validated.</p>
  
 
https://2021.igem.org/wiki/images/a/a3/T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png  
 
https://2021.igem.org/wiki/images/a/a3/T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png  
 
<p><u>Figure</u>. <b>Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning.</b> <i>
 
<p><u>Figure</u>. <b>Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning.</b> <i>
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a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 pB), flagcry11Aa (expected 1847 pB), strepcyt1Aa (expected 775 pB) and 6hisp20 (expected 568 pB); L (SMART Ladder, its size is shown on the right side). </i></p>
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a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 bp), flagcry11Aa (expected 1847 bp), strepcyt1Aa (expected 775 bp) and 6hisp20 (expected 568 bp); L (SMART Ladder, its size is shown on the right side). </i></p>
  
  

Latest revision as of 08:56, 21 October 2021


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Applications of BBa_K3788001

PCR amplification of flagCry11Aa gene :

The DNA sequence of flagcry11Aa was amplified by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is 1956bp and a 2000bp band is observed. Amplification of DNAs sequences with primers was validated.

T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png

Figure. Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning. a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 bp), flagcry11Aa (expected 1847 bp), strepcyt1Aa (expected 775 bp) and 6hisp20 (expected 568 bp); L (SMART Ladder, its size is shown on the right side).


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