Difference between revisions of "Part:BBa K3733005"
 
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LThe strength of this promoter is much weaker than J23106. </p>
 
LThe strength of this promoter is much weaker than J23106. </p>
  
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===Usage and Biology===
 
===Usage and Biology===
 
<p>J23106 is a medium-strength constitutive promoter of the Anderson family. By editing J23106's -35 region by replacing nucleotides 3-5 from TAC to ATA, J23106a was successfully constructed (<b>Figure 1</b>)，which is a very weak promoter. </p>
 
<p>J23106 is a medium-strength constitutive promoter of the Anderson family. By editing J23106's -35 region by replacing nucleotides 3-5 from TAC to ATA, J23106a was successfully constructed (<b>Figure 1</b>)，which is a very weak promoter. </p>
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<center><img src="https://static.igem.org/mediawiki/parts/9/99/T--HZAU-China--J23106a_1.png" style="width:793px;height:360px"></center>
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<center><img src="https://static.igem.org/mediawiki/parts/9/99/T--HZAU-China--J23106a_1.png" style="width:640px;height:360px"></center>
 
<center><b>Figure 1.</b>Modified methods of J23106a</center>
 
<center><b>Figure 1.</b>Modified methods of J23106a</center>
 
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===Functional Parameters===
 
===Functional Parameters===
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<center><img src="https://static.igem.org/mediawiki/parts/5/51/T--HZAU-China--J23106a_2.png" style="width:793px;height:360px"></center>
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<center><img src="https://static.igem.org/mediawiki/parts/5/51/T--HZAU-China--J23106a_2.png" style="width:692px;height:530px"></center>
 
<center><b>Figure 2.</b>Comparison of relative strength between J23106 and J23106a.The microplate reader gain adopts 50</center>
 
<center><b>Figure 2.</b>Comparison of relative strength between J23106 and J23106a.The microplate reader gain adopts 50</center>
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3733005 parameters</partinfo>
 
<partinfo>BBa_K3733005 parameters</partinfo>
 
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Latest revision as of 14:33, 15 October 2021


J23106a:Improved promoter of J23106

J23106a is a constitutive promoter obtained by mutating J23106.（https://parts.igem.org/Part:BBa_J23106） LThe strength of this promoter is much weaker than J23106.


Usage and Biology

J23106 is a medium-strength constitutive promoter of the Anderson family. By editing J23106's -35 region by replacing nucleotides 3-5 from TAC to ATA, J23106a was successfully constructed (Figure 1)，which is a very weak promoter.

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Figure 1.Modified methods of J23106a


Functional Parameters

To characterize this part, J23106a and J23106 were cloned into pSC101 vector separately. We chose neGFP（https://parts.igem.org/Part:BBa_K3733012） as the reporter. Plasmids were transferred into E.coli DH5α. The E. coli strain was cultured overnight, and the relative intensity of J23106a was evaluated by measuring RFU/OD600 under the control of two different promoters. The relative fluorescence unit (RFU) was determined by Synergy H1 microplate reader. 

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Figure 2.Comparison of relative strength between J23106 and J23106a.The microplate reader gain adopts 50

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

biologyEscherichia coli