Difference between revisions of "Part:BBa E0051:Design"
 
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===Design Notes===
 
===Design Notes===
 +
LacZa was placed first in discussion with Joey Davis (Sauer lab). The subunit interfaces of lacZ are at the N-terminal residues and also lacZa is shorter and doesn't have any potential internal translation start sites wheras GFP might have some in-frame RBS and ATG which could possibly lead to translation of lacZa without GFP if GFP were first.
 +
 
The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc. The start codon from GFP was removed.
 
The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc. The start codon from GFP was removed.
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−
 
  
 
===Source===
 
===Source===

Latest revision as of 20:58, 3 March 2009

lacZa.GFP fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 878


Design Notes

LacZa was placed first in discussion with Joey Davis (Sauer lab). The subunit interfaces of lacZ are at the N-terminal residues and also lacZa is shorter and doesn't have any potential internal translation start sites wheras GFP might have some in-frame RBS and ATG which could possibly lead to translation of lacZa without GFP if GFP were first.

The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc. The start codon from GFP was removed.

Source

amino acids 1-60 from lacZ and amino acids 2-end of GFP (BBa_E0043)

References