Difference between revisions of "Part:BBa K3745035"

Latest revision as of 10:41, 14 October 2021

LacI promoter and LacI

This part encodes LacI promoter and LacI, which could express repressor protein to bind with lac operator and repress transcription. However, when lactose or IPTG is present, the repressor will first combine with lactose or IPTG, and could no longer restrict the transcription of genes downstream of the operon.

In our project, it is used to control the expression on rhlA,B,C, and protein scaffold.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3745035 short</partinfo>
-This part encodes rhlA,B,C as well as tags for each enzyme and scaffold to connect these three enzymes together, and it is the most essential plasmid in our protein scaffold project. We place the ORF of specific tags in front of each enzyme's ORF. These tags could bind with the corresponding domain on the scaffold and form a protein complex that can carry out the three needed reactions to synthesize rhamnolipid.
+This part encodes LacI promoter and LacI, which could express repressor protein to bind with lac operator and repress transcription. However, when lactose or IPTG is present, the repressor will first combine with lactose or IPTG, and could no longer restrict the transcription of genes downstream of the operon.
-We adopt the ratio of each domain on the scaffold from "", we plan to fine-tune the ratio of the domain in the future.
-By connecting rhlA,B,C together, we successfully improved the reaction efficiency of the enzymes that assemble rhamnolipid by maximizing the collision probability of substrates and enzymes as well as minimizing the time interview between each reaction, hence increase rhamnolipid yield.
-For the detailed function of each enzyme, see BBa_K3745030
+In our project, it is used to control the expression on rhlA,B,C, and protein scaffold.